浙江农林大学学报2017，Vol.34Issue(2)：332-339,8.DOI:10.11833/j.issn.2095-0756.2017.02.018
菠萝EST资源的SSR信息分析与开发
Development of novel EST-SSR markers in pineapple (Ananas comosus) by data mining from public EST sequences
摘要
Abstract
Expressed sequence tags (ESTs) are used for microsatellite development. EST derived from simple sequence repeats (EST-SSRs), one of the most common new microsatellite markers, are more likely to be em-bedded in functional gene sequences, to be less costly and time effective, and may provide more information. In the present study, analysis of 5659 publicly available expressed sequence tags of pineapple (Ananas comosus) was conducted with the Webstat online program. Results of 636 (SSRs), identified electronically in 617 ESTs, showed that the average density was one SSR per 7.39 kb of ESTs screened. Dinucleotide (42.61%), trinu-cleotide (29.25%), and hexanucleotide (20.13%) repeats were the dominant types among the obtained motifs. The least frequent was tetranucleotide repeats (3.46%). TC/AG was most abundant in the dinucleotide motif. Of the 30 functional pairs, 27 primer pairs showed polymorphisms by polymerase chain reaction (PCR) ampli-fications. Thus, the EST-SSRs developed here with the first large-scale development of SSR markers in pineap-ple have the potential to be used for development of genetic and physical maps, quantitative trait loci mapping, genetic diversity studies, association mapping, and finger printing cultivars.关键词
分子生物学/菠萝/表达序列标签/简单重复序列Key words
molecular biology/Ananas comosus/ESTs/SSR分类
农业科技引用本文复制引用
陈俊浩,陆洋,倪燕婕,张智俊,钟雅萍,鲍晶晶,罗淑萍..菠萝EST资源的SSR信息分析与开发[J].浙江农林大学学报,2017,34(2):332-339,8.基金项目
浙江省自然科学基金资助项目(LY14C160009) （LY14C160009）
