掺Mg与接枝RGD的HAnps载体系统对MG63细胞胞吞功能的影响
Influence of HAnps carrier system with doping Mg and grafting RGD on endocytosis of MG63 cells
摘要
Abstract
HAnps and HAnps with doped Mg (Mg-HAnps) were prepared by hydrothermal synthesis method (HTSM), grafted adhesive peptide (RGD) after silanization combined with carbodiimide method, labeled HAnps, Mg-HAnps, RGD-HAnps, RGD-Mg-HAnps with coumarin-6 as a fluorescent label, and they were co-cultured with human osteosarcoma MG63 cell line (MG63) cells. The effects of HAnps with doping Mg and grafting RGD on endocytosis of MG63 cells were investigated. The results show that RGD-Mg-HAnps have no cytotoxicity. Mg-doped and RGD-grafted HAnps improve endocytosis of MG63 cells, which have synergistic effects. RGD-Mg-HAnps are able to promote endocytosis.关键词
基因载体/掺Mg羟基磷灰石纳米颗粒(Mg-HAnps)/黏附肽(RGD)/靶向性/胞吞作用Key words
gene vector/Mg-doped Hydroxyapatite nanoparticles (Mg-HAnps)/arginine-glycine-aspartic acid (RGD)/targeting specificity/endocytosis分类
医药卫生引用本文复制引用
陈恬,陈良建,贺惠莉,彭静,郭筱慧,郑雅亦,邵春生..掺Mg与接枝RGD的HAnps载体系统对MG63细胞胞吞功能的影响[J].中南大学学报（自然科学版）,2017,48(3):625-634,10.基金项目
国家自然科学基金资助项目(81571021) （81571021）
湖南省科技重点项目(2015WK3012) （2015WK3012）
中南大学湘雅三医院新湘雅人才项目(20160301) （20160301）
湖南省高层次卫生人才"225"工程骨干人才项目(225) （225）
中南大学本科生自由探索计划项目(ZY2016758) （ZY2016758）
中南大学粉末冶金国家重点实验室资助项目(621020094) (Project(81571021) supported by the National Natural Science Foundation of China （621020094）
Project (2015WK3012) supported by the Hunan Provincial Science and Technology Department Program （2015WK3012）
Project(20160301) supported by the New Xiangya Talent Program of the Third Xiangya Hospital of Central South University （20160301）
Project(225) supported by the High Level Health Personnel in Hunan Province, China （225）
Project(ZY2016758) supported by the Undergraduate Free Exploration Program in Central South University （ZY2016758）
Project (621020094) supported by State Key Laboratory of Powder Metallurgy in Central South University) （621020094）
