中南民族大学学报（自然科学版）2017，Vol.36Issue(1)：37-42,6.
青蒿提取物舒张小鼠气管平滑肌的作用机理
Relaxant Action of Artemisia Annua L.Extract on Mouse Airway Smooth Muscle
摘要
Abstract
Water extract of Artemisia Annua L.(WEAA) was prepared and used to survey the relaxant effect on mouse airway smooth muscle.In order to investigate it, high K+ or ACh was employed, which was a specific activator of L-type Ca2+ channel(VDCC)or M-Receptor, respectively.Firstly, a dose-dependent relaxant manner was presented on high K+ and ACh-induced pre-contracted mouse tracheal rings when treated with WEAA, and they were relaxed completely in 10 mg/mL of WEAA.However, no effect was examined on resting tension.VDCCs were completely blocked and Ca2+ influx was inhibited in both high K+ and ACh-induced pre-contracted ASM.These results suggest that other membrane channels might be also involved in this process.Secondly, the intracellular Ca2+ release was inhibited, and no significant effect was found on contractility of mouse tracheal rings after the treatment of WEAA.The data demonstrate that WEAA resulted in relaxation of pre-contracted ASM by blocking VDCC and other membrane channels to inhibit Ca2+ influx and intracellular Ca2+release.This study indicates that WEAA would be a potential bronchodilators for asthma.关键词
青蒿提取物/气管平滑肌/哮喘Key words
Artemisia Annua L.extract/airway smooth muscle/asthma分类
生物科学引用本文复制引用
沈金花,黄俊..青蒿提取物舒张小鼠气管平滑肌的作用机理[J].中南民族大学学报（自然科学版）,2017,36(1):37-42,6.基金项目
国家自然科学基金资助项目(81170227) （81170227）
湖北省自然科学基金重点资助项目(2012FFA028) （2012FFA028）
