作物学报2017，Vol.43Issue(4)：473-482,10.DOI:10.3724/SP.J.1006.2017.00473
水稻叶片淀粉积累及早衰突变体esl9的鉴定与基因定位
Identification and Gene Mapping of Starch Accumulation and Early Senes-cence Leaf Mutantesl9 in Rice
摘要
Abstract
A new leaf senescence mutantesl9(early senescence leaf 9) was discovered from the progeny of indica maintainer line 1B mutated by ethyl methane sulfonate(EMS). Compared with the wild-type, the leaf ofesl9 was pale green at seedling stage; chlorosis occurred at leaf tip and gradually extended to the middle-upper parts of leaf at tillering stage. However, the leaf base remained green until maturity. In the esl9, the photosynthetic pigment contents declined, and the contents of reactive oxygen spe-cies (ROS), such as O2ˉ,·OH, and H2O2, heightened compared with those in the wild-type. At the same time, activities of protec-tive enzymes, SOD and CAT, both reduced inesl9. The results of iodine-iodide kalium dyeing and starch content determination showed that more starch granules accumulated in theesl9leaf. Quantitative RT-PCR results indicated that genes responsible for starch synthesis were up-regulated and genes participated in the triose phosphate distribution path were down-regulated. We made an inference that gene mutation changed the distribution of triose phosphate, resulting in starch granules accumulating in the leaf, chloroplast structure being destroyed and photosynthetic system being blocked, thus increasing the contents of ROS, eventually causing leaf senescence. Genetic analysis demonstrated that the phenotype ofesl9 was controlled by a dominant nuclear gene. The target gene ESL9 was mapped between SSR markers S11-110 and S11-87 with a physical distance of 304.9 kb on chromosome 11. These results will lay a foundation for cloning and functionally analysingESL9.关键词
水稻(OryzasativaL.)/早衰/淀粉积累/遗传分析/基因定位Key words
Rice (Oryza sativa L.)/Early senescence/Starch accumulation/Genetic analysis/Gene mapping引用本文复制引用
肖艳华,何光华,陈新龙,杜丹,邢亚迪,张天泉,祝毛迪,刘明明,朱小燕,桑贤春..水稻叶片淀粉积累及早衰突变体esl9的鉴定与基因定位[J].作物学报,2017,43(4):473-482,10.基金项目
本研究由农业部行业专项(201303129)和重庆市重点实验室能力提升项目(cstc2014pt-sy80001)资助. This study was supported by the Special Industry Project of Ministry of Agriculture (201303129) and the Capacity Promotion Project of Key Laboratories in Chongqing (cstc2014pt-sy80001). （201303129）
