作物学报2017，Vol.43Issue(4)：483-495,13.DOI:10.3724/SP.J.1006.2017.00483
小麦茎秆断裂强度相关性状QTL的连锁和关联分析
Linkage Analysis and Genome-Wide Association Study of QTLs Controlling Stem-Breaking-Strength-Related Traits in Wheat
摘要
Abstract
Stem strength has close relationship with lodging character, thereby, affects final yield in wheat. The objectives of this study were to unravel the genetic mechanism of stem-breaking-strength-related traits and find molecular markers closely linked or associated with these traits. We tried to map the stem-breaking-strength-related QTLs through linkage analysis using the RIL population consisting of 173 F8:9 lines derived from Shannong 01-35× Gaocheng 9411) and association analysis using a nature population consisting of 2015 wheat varieties. Both populations were planted in two environments and genetically screened with the 90 k SNP array, DArT technology, and traditional molecular markers. By means of the existing high-density genetic map, nine additive QTLs were detected in different regions on chromosome 4B, such as DURUM_CONTIG63670_287–IACX557 and EX_C101685–RAC875_C27536, which controlled stem-breaking strength, plant height, filling degree of the second internode and culm wall thickness of the second internode and explained 9.40%–36.30% of the phenotypic variations. By means of a composite map (containing 24355 SNPs) based on the Illumina Infinium assay, a total of 37 SNPs were found in the natural population to be associated with stem-breaking-strength-related traits (P < 0.0001). These SNPs were distributed on chromosomes 1B, 2B, 2D, 3A,3B, 4B, 5A, 5D, 6B, 7A, 7B, and 7D and explained 7.76%–36.36% of the phenotypic variations. The genetic distance between RAC875_C27536 detected through linkage analysis and Tdurum_contig4974_355 detected through genome-wide association analysis was 6.7 cM in the composite map, indicating the presence of important genes controlling stem strength in this region.关键词
普通小麦/QTL定位/连锁分析/全基因组关联分析/茎秆断裂强度Key words
Common wheat/QTL mapping/Linkage analysis/Genome-wide association study (GWAS)/Stem-breaking strength引用本文复制引用
刘凯,陈建省,邓志英,张莹,王芳芳,刘佟佟,李青芳,邵文,赵宾,田纪春..小麦茎秆断裂强度相关性状QTL的连锁和关联分析[J].作物学报,2017,43(4):483-495,13.基金项目
本研究由山东省自然科学基金项目(2015ZRB01179), "小麦重要品质性状形成和改良的分子基础"育种专项和山东省种质资源创制课题资助. This study was supported by the Natural Science Foundation of Shandong Province, China (2015ZRB01179), the breeding project of "Molecular Foundation for Formation and Improvement of Main Quality Traits of Wheat", and the Shandong Provincial Project for Germplasm Resource Innovation. （2015ZRB01179）
