作物学报2017，Vol.43Issue(4)：522-529,8.DOI:10.3724/SP.J.1006.2017.00522
水稻淡黄叶矮化突变体yld的遗传分析及基因定位
Genetic Analysis and Gene Mapping ofYellow leaf and dwarf (yld)Mutantin Rice
摘要
Abstract
Leaf color mutants of rice are ideal materials in studies on photosynthesis, chlorophyll metabolism and chloroplast development in plants. A yellow leaf and dwarf mutant,Yellow leaf and dwarf(yld),was obtained from ethyl methane sulfonate (EMS)-treated Shuhui 527 (Oryza sativa L.). Compared with the wild type, theyld mutant showed yellow leaf and dwarfism, and the contents of chlorophyll and carotenoid were obviously decreased. Transmission electron microscope observation revealed that the structure of most chloroplasts seemed to be normal, however, with the fuzzy grana, and fewer and looser stroma lamella in the yld mutant. Meantime, plant height, branch number, grain number per panicle, 1000-grain weight and seed-setting rate were sig-nificantly decreased, while the number of effective panicle was obviously increased in theyld mutant compared with those in the wild type. Genetic analysis showed that the yld mutant traits were controlled by a single recessive gene. The 323 mutational indi-viduals from the F2 generation of the cross of Wuyunjing 7 and yld mutant were used for gene mapping. Finally, theYLDlocus was mapped on chromosome 11 between two Indel markers L5 and L7, with an approximate 115 kb physical region. These results would facilitate cloning and functional analysis for the YLD gene.关键词
水稻/淡黄叶矮化突变体/遗传分析/基因定位Key words
Rice/Yellow leaf and dwarf mutant/Genetic analysis/Gene mapping引用本文复制引用
李自壮,徐乾坤,余海平,周亭亭,薛大伟,曾大力,郭龙彪,钱前,任德勇..水稻淡黄叶矮化突变体yld的遗传分析及基因定位[J].作物学报,2017,43(4):522-529,8.基金项目
本研究由国家自然科学基金项目(31401464)资助. The study was supported by the National Natural Science Foundation of China (31401464). （31401464）
