作物学报2017，Vol.43Issue(4)：530-548,19.DOI:10.3724/SP.J.1006.2017.00530
利用荧光SSR分析中国糜子遗传多样性
Analysis of Genetic Diversity in Common Millet (Panicum miliaceum) Using Fluorescent SSR in China
摘要
Abstract
Understanding of the genetic diversity of common millet germplasm resources is a basis of research on its origin and evolution, as well as the effective utilization of elite germplasm. In this study, we analyzed the genetic diversity of 132 common millet accessions collected from 11 provinces of China using 15 millet-specific fluorescent-labelled simple sequence repeat (SSR) markers. A total of 107 alleles were detected with 7 alleles per locus ranging from 2 to 14. The expected heterozygosity ranged from 0.0936 to 0.8676 with the mean value of 0.5298. The polymorphism information content (PIC) ranged from 0.0893 to 0.8538 with the mean value of 0.4864. The 132 accessions were clustered into four groups according to genetic distance between acces- sions, which showed geographic and eco-regional distributions. Group I is a collection of germplasm from spring-sowing area of Northeast China, group II is composed of partial accessions from the spring and summer-sowing areas of Loess Plateau, group III is composed of partial accessions from the spring-sowing area of Northern China, and the remaining accessions comprises Group IV with geographic overlaps to groups II and III. Model-based genetic structure analysis indicated that the genetic resources of Chinese common millet were from four gene pools in Northeast, Loess Plateau, Northern, and Northwest China. Obviously, this result was very similar to that of clustering analysis, showing the importance of geographical relationship. Rich genetic variations were also found in Chinese common millet resources, mainly from different accessions. The above results provide an overall evaluation of genetic diversity of common millet in China at a molecular level.关键词
糜子/荧光SSR/遗传多样性/聚类分析/遗传结构Key words
Common millet (Panicum miliaceum L.)/Fluorescent SSR/Genetic diversity/Cluster analysis/Genetic structure引用本文复制引用
王瑞云,季煦,陆平,刘敏轩,许月,王纶,王海岗,乔治军..利用荧光SSR分析中国糜子遗传多样性[J].作物学报,2017,43(4):530-548,19.基金项目
本研究由国家自然科学基金项目(31271791), 山西省回国留学人员科研资助项目(2016-066), 国家自然科学基金青年科学基金(31301386), 国家自然科学基金青年科学基金(31300279), 国家现代农业产业技术体系建设专项(CARS-07-13.5-A12), 山西省重点研发计划(一般项目)(农业)项目(201603D221003-5)资助. This study was supported by the National Natural Science Foundation of China (31271791), the Scientific Research Foundation for Returned Scholars in Shanxi province (2016-066), the National Natural Science Foundation of China for Young Scientists (31301386, 31300279), the China Agriculture Research System (CARS-07-13.5-A12), and General Project of the Shanxi Provincial Key Research and Development Program for Agriculture (201603D221003-5). （31271791）
