植物学报2017，Vol.52Issue(2)：218-224,7.DOI:10.11983/CBB16033
氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响
Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata
摘要
Abstract
We investigated the effects of amino acid supplements on thallus growth,superoxide dismutase (SOD) activity and huperzine-A accumulation in Huperzia serrata by adding aspartic acid,lysine,and tryptophan at different concentrations into the medium.With aspartic acid,the dry weight of the thallus increased by 18.50％-25.75％ and the huperzine-A accumulation was significantly higher than in the control.The optimal concentration of aspartic acid was 0.5 mmol·L-1.The relative growth of thallus was significantly higher with 1 mmoI·L-1 tryptophan than in the control,but the huperzine-A accumulation was slightly lower than for the control.The relative growth rate,dry weight and huperzine-A accumulation was lower for thallus cultured with lysine than the control.The SOD activity of the thallus cultured with aspartic acid was increased properly,and the huperzine-A accumulation of the thallus was increased.However,huperzine-A accumulation of the thallus was suppressed when SOD activity remained high in the long-term.关键词
蛇足石杉/石杉碱甲/前体物/SOD酶Key words
Huperzia serrata/huperzine A/precursor/superoxide dismutase引用本文复制引用
陈曼,涂艺声,叶丽婻,杨碧芸..氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响[J].植物学报,2017,52(2):218-224,7.基金项目
国家自然科学基金(No.81360614,No.31370390,No.81660597)、江西省自然科学基金(No.20132BAB204023)和江西省科技支撑项目(No.20121BBG70016) （No.81360614,No.31370390,No.81660597）
