植物营养与肥料学报2017，Vol.23Issue(1)：54-61,8.DOI:10.11674/zwyf.16126
三种硝化抑制剂抑制土壤硝化作用比较及用量研究
Inhibition of DCD, DMPP and Nitrapyrin on soil nitrification and their appropriate use dosage
摘要
Abstract
[Objectives] Nitrification inhibitors are effective in regulating the transformation of soil nitrogen and the community structure of microbes which involve in the N nitrification process.Incubation and pot experiments were conducted to compare nitrification effects of 3 common used nitrification inhibitors,and to study the dosage for practical use in calcareous soil.[Methods] The incubation experiment was carried out in a plant growth chamber at 25 ℃ in dark condition,and the pot experiment was conducted in greenhouse.The tested three inhibitors were dicyandiamide (DCD),3,4-dimethylpyrazole phosphate (DMPP) and chloro-6-trichloromethyl-pyridine (nitrapyrin).The dosage of DCD and DMPP designed as 0 (CK),1.0％,2.0％,3.0％,3.5％,4.0％,4.5％,5.0％,6.0％ and 7.0％ of the N addition,those of nitrapyrin were 0,0.1％,0.125％,0.2％,0.25％,0.3％,0.35％,0.4％,0.45％ and 0.5％.N 0.50 g per kg of dry soil was added in pot experiment,and the dosages of three inhibitors were all 5％,1％,0.648％ of the N addition.The nitrate inhibition rate and the biomass of pakchoi were investigated;the soil microbial community structures of AOA and AOB were analyzed using the method of denaturing gradient gel electrophoresis (DGGE).[Results] Soil nitrification was significantly inhibited by DCD,DMPP and nitrapyrin (P ＜ 0.05).Compares with the CK,soil NH4+-N concentrations were increased by 46.2-256.1 mg/kg with DCD,by 291.8-376.7 mg/kg with DMPP and by 3.68-372.9 mg/kg with nitrapyrin,respectively.The nitrification inhibition rates were 49.3％-79.4％ with DCD and 96.4％-99.4％ with DMPP.The DCD addition exhibited an obvious dose effect,namely,the nitrification inhibition rates were increased significantly with DCD dose increasing,the DMPP did not in the addition range of 1.0％-7.0％,Although nitrapyrin only showed dose response in low concentrations from 0.1％ to 0.2％,the inhibition rate had reached 98.9％-99.9％ when nitrapyrin concentration raised over 0.25％.The inhibition ability followed the order of nitrapyrin ≥ DMPP ＞ DCD.A significant impact of all three NIs on AOA and AOB community structure alteration was found,but there was no significant difference among the three inhibitors.The pakchoi above-ground weight,the contents of Vitamin C and amino acid were significantly improved by DCD,DMPP and nitrapyrin addition (P ＜ 0.05),with the increase of pakchoi biomass of 12.7％ with DCD,11.1％ with DMPP and 17.6％ with nitrapyrin,respectively.[Conclusions] Briefly,DCD,DMPP and nitrapyrin showed significant role in retarding nitrification in calcareous soil and exerted an obvious influence on AOA and AOB community structure (P ＜ 0.05).Comparatively,nitrapyrin was superior over DMPP and DCD on the soil inhibition,and the addition dose of 0.25％ based on pure N was recommended for nitrapyrin application.关键词
硝化抑制剂/石灰性土壤/铵态氮/硝态氮/硝化抑制率/氨氧化古菌/氨氧化细菌Key words
nitrification inhibitor/calcareous soil/ammonium/nitrate/nitrification inhibition rate/AOA/AOB引用本文复制引用
王雪薇,刘涛,褚贵新..三种硝化抑制剂抑制土壤硝化作用比较及用量研究[J].植物营养与肥料学报,2017,23(1):54-61,8.基金项目
国家“十二五”科技支撑项目(2012BAD42B02)资助. （2012BAD42B02）
