中药材2016，Vol.39Issue(10)：2173-2177,5.DOI:10.13863/j.issn1001-4454.2016.10.001
山西黄芪根腐病优势病原菌鉴定及其拮抗菌的多重筛选
Identification of Dominant Pathogen Causing Root Rot Disease in Astragalus membranaceus var.mongholicus of Shanxi Province and Screening of Antagonistic Microorganism
摘要
Abstract
Objective:To identify the dominant pathogen causing Astragalas membranaceus vat.mongholicus root rot disease and to screen the antagonistic microorganism.Methods:Pathogens were isolated from diseased root segments and the pathogenicity was tested by using Koch's postulate.Morphological study and EF-1α molecular identification were applied to determine the classification status.At the same time,the dominant pathogen was as the target,bio control strains were selected by multiple screening in vitro.Results:Fusarium solani was one of the dominant pathogens which causing root rot disease in Astragalas membranaceus var.mongholicus of Shanxi province.The screened antagonistic strain G10 showed significant effect of inhibiting Fusarium solani and controlling Astragalas membranaceus var.mongholicus root rot disease in vitro.Conclusion:The antagonistic strain G10 against Fusarium solani has a great potential for development as a commercial bio-control agent.关键词
蒙古黄芪/根腐病/病原鉴定/拮抗菌/多重筛选Key words
Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao/Root rot disease/Identification of pathogen/Antagonistic microorganism/Multiple screening分类
医药卫生引用本文复制引用
任小霞,秦雪梅,王梦亮,雷振宏,王玉龙,高芬..山西黄芪根腐病优势病原菌鉴定及其拮抗菌的多重筛选[J].中药材,2016,39(10):2173-2177,5.基金项目
山西省中药现代化关键技术研究专项(2014ZD0501-2) （2014ZD0501-2）
