中药材2016，Vol.39Issue(10)：2268-2271,4.DOI:10.13863/j.issn1001-4454.2016.10.028
一测多评法测定白屈菜中5种生物碱的含量
Determination of Five Alkaloids from Chelidonium majus by HPLC-QAMS
黄涛阳 1王晖 2翁燕君 1谢新民 1黄和 1谢秋玲1
作者信息
- 1. 广州军区广州总医院药剂科,广东广州510010
- 2. 广州军区联勤部药品仪器检验所,广东广州510500
- 折叠
摘要
Abstract
Objective:To establish a method of quantitative analysis of multi-components by single marker(QAMS) for simultaneous determining five alkaloids in Chelidonium majus.Methods:Coptisine was used as the internal reference standard,the relative correction factors of chelerythrine,chelidonine,sanguinarine,berberine were calculated.The contents of remaining ingredients were calculated by using relative correction factors.At the same time,the external standard method was used to detect the contents of five alkaloids,the differences of the two methods were compared,and the accuracy and feasibility of QAMS were verified.Results:The results calculated with quantitative analysis of multi-components by QAMS were consistent with the results by the external standard method,and there were no significant differences between the quantitative results of the QAMS and external standard method.Conclusion:HPLC-QAMS can be used for the quality evaluation of Chelidonium majus.关键词
一测多评法/白屈菜/相对校正因子Key words
Quantitative analysis multi-components by single marker(QAMS)/Chelidonium majus L./Relative correction factor (RCF)分类
医药卫生引用本文复制引用
黄涛阳,王晖,翁燕君,谢新民,黄和,谢秋玲..一测多评法测定白屈菜中5种生物碱的含量[J].中药材,2016,39(10):2268-2271,4.
