中国现代中药2017，Vol.19Issue(2)：165-173,9.DOI:10.13313/j.issn.1673-4890.2017.2.002
HepG2细胞胰岛素抵抗模型的建立及在筛选两色金鸡菊活性化合物中的应用
Establishment of Insulin Resistance HepG2 Cell Model and Its Application in Screening Bioactive Components of Coreopsis tinctoria
摘要
Abstract
Objective:To establish human hepatoma carcinoma cell (HepG2 cell)model of insulin resistance in vitro and to initially select the effective ingredients of Coreopsis tinctoria.Methods:HepG2 cells were induced by insulin,palmic acidor glucose of different concentrations for different time.Through the estimation of the cell activity with MTT method and glucose consumption with glucose oxidase method,the effective concentrations and time of inducer in the insulin resistance cell model were ascertained.After the cell model establishment,HepG2 cells were pretreated with crude extracts of C.tinctoria,different fractions or compounds of different concentrations for 24 h,and the influences on the model cells regarding glucose consumption were observed through the glucose oxidase method or fluorescent glucose 2-NBDG and the cell viability was evaluated by the MTT method.Results:Compared with the control group,1 × 10-7 mol · L-1 insulin-stimulated cells 24 h or 55 mmol· L-1 glucose-stimulated cells 24 h has a significant insulin resistance effect,and high concentrations of insulin or glucose did not affect the cell viability,while palmitic acid group emerges insulin resistant but the cell viability was also significantly decreased.Screening results showed that the 5th compound falavanomarein and the 6th compounds marein had better preventive activity of high glucose-induced insulin resistance.Conclusion:Insulin resistance cell models are successfully made.Falavanomarein and marein could be the main active compounds of C.tinctoria.关键词
两色金鸡菊/胰岛素抵抗/HepG2细胞Key words
Coreopsis tinctoria/insulin resistance/HepG2 cell引用本文复制引用
姜保平,乐亮,姚霞,许利嘉,肖伟,肖培根..HepG2细胞胰岛素抵抗模型的建立及在筛选两色金鸡菊活性化合物中的应用[J].中国现代中药,2017,19(2):165-173,9.基金项目
国家自然科学基金资助项目(81274188) （81274188）
中国医学科学院医学与健康科技创新工程经费资助(81573576) （81573576）
