中国现代中药2017，Vol.19Issue(2)：174-182,9.DOI:10.13313/j.issn.1673-4890.2017.2.003
HPLC-Q-TOF/MS鉴定白屈菜中异喹啉类生物碱
Identification of Isoquinoline Alkaloids in Chelidonium majus by HPLC-Q-TOF/MS
摘要
Abstract
Objective:To systematically and rapidly identify isoquinoline alkaloids in Chelidonium majus using the HPLC-Q-TOF/MS.Methods:The separation was performed on a XAqua Cs column(150 mm × 2.1 mm,5 pn)with acetonitrile-0.1％ formic acid in water by gradient elution.The flow rate was 0.3 mL· min-1.Electrospray ionization-(ESI) source was applied and operated in positive ion mode.The structure of alkaloids was speculated by MS/MS fragmentation behaviors of standards and related references.Results:18 alkaloids were elucidated from the methanolic extracts of Ch.majus.Compounds magnocurarine (1),canadine (4),jatrorrhizine (12),columbamine (14) were reported firstly in Ch.majus.Conclusion:HPLC-Q-TOF/MS is rapid and reliable method for identification of the alkaloids in Ch.majus.The results would provide scientific evidence for further extraction and separation of components and study of pharmacodynamic material basis in Ch.majus.关键词
HPLC-Q-TOF/MS/白屈菜/异喹啉类生物碱Key words
Isoquinoline alkaloids/HPLC-Q-TOF/MS/Chelidonium majus L.引用本文复制引用
杨鹏,卿志星,左姿,余坤,曾建国..HPLC-Q-TOF/MS鉴定白屈菜中异喹啉类生物碱[J].中国现代中药,2017,19(2):174-182,9.基金项目
长沙市科技计划重点项目(K1308182-21) （K1308182-21）
