中国现代中药2017，Vol.19Issue(2)：183-187,5.DOI:10.13313/j.issn.1673-4890.2017.2.004
NMR法分析鉴定神曲的发酵产物
Identification of Chemical Component in SHEN QU Based on NMR Analysis
摘要
Abstract
Objective:To identify compounds and analyze the effect of producing area,procedure and ingredient on the components of SHEN QU.Method:1 H-NMR,13 C-NMR,HMBC,1H-1 H COSY and TOCOSY were employed to identify the compounds from SHEN QU,multivariate analysis was applied to find the difference among 36 batch SHEN QU got from different county.Result:In this study,succinic acid(1),lactic acid(2),glyceric acid(3),methoxyacetic acid(4),methoxy acetic acid ammonium(5),fatty acid(6),glycerin(7),long chain olefins(8),α-D-fructose(9),α-D-glucose(10)andβ-D-glucose(11)were elucidated in SHEN QU on the basis of NMR technology,compounds 1-9 were identified for the first time in it.1 H-NMR analysis showed that the chemical component in stock material almost not be detected in end product of SHEN QU,while many new signals of saccharides,fatty acids and long chain alkenos were detected.The result of PLS-DA analysis of 36 batch sample indicated that chemical components of SHEN QU from different region are similar.Conclusion:The fermentation process of SHEN QU is more important factor affecting the chemical components than procedure,producing region and ingredients.关键词
神曲/化学成分/核磁共振技术/多元分析/发酵Key words
SHEN QU/chemical component/NMR/multivariate analysis/fermentation引用本文复制引用
曹美娇,张婷婷,许枬,陈俏,陶鑫,曹佳..NMR法分析鉴定神曲的发酵产物[J].中国现代中药,2017,19(2):183-187,5.基金项目
国家公益性行业专项项目(201507004-03) （201507004-03）
