中国现代中药2017，Vol.19Issue(2)：209-213,5.DOI:10.13313/j.issn.1673-4890.2017.2.010
全蝎质量评价及标准提高研究
Standard Improvement of Scorpion and Quality Evaluation
摘要
Abstract
Objective:To establish the quality standard for scorpion used for quality evaluation.Methods:The contents of moisture,total ash and acid insoluble ash were determined.The effect of different extract solvents on the determination was investigated.Lipid components of scorpion were identified by TLC,the contents of characteristic amino acids were determined by HPLC.Results:The content of moisture,total ash and acid insoluble ash had been measured.The extract determination was more reasonable with ethanol as the solvent.The developed TLC spots were obvious.The method had good linear relationship within the range of 0.509 1-5.091 0 μg for glyeine(r =1.000 0),0.502 5-5.025 0 μg for arginine (r =1.000 0),0.348 2-3.482 0 μg for L-proline(r =1.000 0)and 0.508 8-5.088 0μg for leucine(r =1.000 0).The average recoveries were 102.5％,104.1％,102.5％ and 102.3％ with RSD of 2.7％,2.8％,2.2％ and 2.9％ (n =9),respectively.Conclusion:The method is simple,accurate and specific,and can be used in the quality control of scorpion.关键词
全蝎/检查项/浸出物/TLC/HPLC/质量评价Key words
scorpion/check items/extract/TLC/HPLC/quality evaluation引用本文复制引用
刘凯娜,王晓云,潘赢,程雅婷,庞静,王健,郭金颖..全蝎质量评价及标准提高研究[J].中国现代中药,2017,19(2):209-213,5.基金项目
河北省食品药品监督管理局科技计划项目(ZC2015004) （ZC2015004）
