中国现代中药2017，Vol.19Issue(2)：232-238,242,8.DOI:10.13313/j.issn.1673-4890.2017.2.015
地黄根部响应涝胁迫关键基因的鉴定
Identification of Key Genes in Response to Waterlogging Stress in Root of Rehmannia glutinosa
摘要
Abstract
Objective:We detected differentially expressed genes of Rehmannia glutinosa tuberous root under submergence stress using RNA-seq technique,which would lay foundation on exploring molecular mechanisms of specific response to waterlogging stress for R.glutinosa.Methods:Wen "85-5" was used as material in this study,of which transcriptome was investigated under submergence stress using upgraded digital gene expression profiling (DGE)technique.The expression level was normalized based on Reads Per Kilobase per Million mapped reads (RPKM)method.The differentially expressed genes were selected using | log2 Ratio | ≥ 1 and FDR adjusted p-value of ＜ 0.001 as cutoff,which were performed GO and KEGG pathway analysis.Results:A total of 7249 genes,with 2505 up-regulated(35％)and 4744 down-regulated(65％),were shown different expression after submergence stress.Conclusion:The KEGG enrichment analysis of these differentially expressed genes revealed that numerous genes mapped to "basic metabolism","secondary metabolism","hormone signaling pathways",and "starch and sugar metabolism pathways".The functional analysis suggested that some genes involved in ethanol fermentation pathways and ethylene biosynthesis showed significant up-regulation,and the genes associated with calcium signaling pathways presented express perturbation.In addition,plenty of genes of transcription factors showed differential expression,e.g.,WRKY,GRAS and NAC related to stress presented up-regulation.However,transcription factors modulated development,e.g.,BHLH,MYC,BYB and MADS-box,mainly disclosed down-regulation.It was indicated that submergence stress was severely harmful to plants.关键词
地黄/升级版数字基因表达谱/人工模拟涝害胁迫/差异表达基因/转录因子Key words
Rehmannia glutinosa/upgraded-DGE/artificial submergence stress/differentially expressed genes/transcription factors引用本文复制引用
王翠英,李鑫宇,王潇然,李明杰,张重义,陈新建..地黄根部响应涝胁迫关键基因的鉴定[J].中国现代中药,2017,19(2):232-238,242,8.基金项目
国家自然科学基金项目(30600209) （30600209）
