中国现代中药2017，Vol.19Issue(2)：239-242,4.DOI:10.13313/j.issn.1673-4890.2017.2.016
药用植物短蕊万寿竹的组织培养研究
Study on Tissue Culture of Disporum brachystemon Wang et Tang
摘要
Abstract
Objective:The aim of the research was to develop and utilize Disporum brachystemon Wang et Tang better.Methods:To investigate the effects of different hormone combinations on cluster bud induction and root culture of Disporum brachystemon Wang et Tang.By orthogonal design,stem was used as the explants and the MS was used as the basic culture medium.Results:The results indicated that the best initial medium was MS + 6-BA 1.0 mg· L-1 + NAA 0.2 mg· L-1 + IBA 0.5 mg·L-1.The most effective medium for cluster bud induction was MS + 6-BA 1.5 mg· L-1 + NAA 0.2 mg· L-1 + KT 1.0 mg· L-1,and the multiplication coefficient of cluster bud reached 6.88.The best rooting medium was MS + 6-BA 0.5 mg·L-1 + NAA 2.0 mg· L-1 + AC 2.0 g·L-1,and roofing ratio was above 96％.Conclusion:The tissue culture and propagation system was astablished and solve the shortage of large-scale planting.关键词
短蕊万寿竹/组织培养/丛生芽/生根培养Key words
Disporum brachystemon Wang et Tang/tissue culture/cluster bud induction/rooting culture引用本文复制引用
王一诺,秦双双,黄宝优,韦莹,李翠,韦坤华..药用植物短蕊万寿竹的组织培养研究[J].中国现代中药,2017,19(2):239-242,4.基金项目
国家中医药行业科研专项(201507002),国家自然科学基金(81473309),广西科学研究与技术开发计划项目(桂科合14125008-2-21,桂科重14124002-6,桂科重14124002-1),广西博士后专项资金(2015年) （201507002）
