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响应面法优化莲子蛋白提取工艺研究

孙天霞 尹翌秋 张鹏飞 王敏 张丽轩 赵雨 王思明

中国现代中药2017,Vol.19Issue(2):250-255,6.
中国现代中药2017,Vol.19Issue(2):250-255,6.DOI:10.13313/j.issn.1673-4890.2017.2.019

响应面法优化莲子蛋白提取工艺研究

Optimization of Extraction Process of Proteinfrom Lotus Seed by Response Surface Methodology

孙天霞 1尹翌秋 2张鹏飞 1王敏 1张丽轩 1赵雨 1王思明1

作者信息

  • 1. 长春中医药大学中医药与生物工程研究开发中心,吉林长春130117
  • 2. 长春金赛药业有限责任公司,吉林长春 130012
  • 折叠

摘要

Abstract

Objective:To optimize the extraction process for protein from lotus seed.Methods:Based on the single factor analysis,the lotus seed protein extraction conditions were optimized by the response surface method.Results:The results showed that the optima extraction conditions were as follows:initial pH 8.0,solid-liquid ratio 1∶15,extraction time 7 h,which resulted in in an extraction yield of 14.53%.Conclusion:By using the response surface methodology extraction technology,the extraction process was easy to operate and accurate and good reproducible and has a practical application value.

关键词

莲子/蛋白质/提取条件/响应面法

Key words

Lotus seed/protein/extraction condition/extraction technology

引用本文复制引用

孙天霞,尹翌秋,张鹏飞,王敏,张丽轩,赵雨,王思明..响应面法优化莲子蛋白提取工艺研究[J].中国现代中药,2017,19(2):250-255,6.

基金项目

吉林省中药生物技术重点实验室(20140622003JC) (20140622003JC)

中国现代中药

OACSTPCD

1673-4890

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