中国现代中药2017，Vol.19Issue(3)：358-361,414,5.DOI:10.13313/j.issn.1673-4890.2017.3.011
珠子参药材中皂苷类成分的薄层色谱及HPLC特征图谱研究
Study on TLC and HPLC Fingerprints of Saponin Constituents from the Roots of Panax japonicus var.major
摘要
Abstract
Objective:To establish and improve the TLC and HPLC fingerprint methods for identification of the roots of Panax japonicus var.major.Methods:The TLC method was adopted to distinguish saponin constituents of the roots of P.japonicus var.major.A separation was performed on a Waters XTerra MS C18 chromatographic column(250 mm×4.6 mm,5 μm)with gradient elution.The mobile phase consisted of acetonitrile-phosphoric acid water solution(0.2%).The analysis time was 55 min and the detection wavelength was 203 nm.Results:The TLC separation degree was good,the separated spots were clear with specific attribute,and the HPLC fingerprints of saponin constituents of the roots of P.japonicus var.major was set up.Conclusion:The TLC and HPLC fingerprint methods are stable,accurate,reliable,and can provide a scientific basis for the quality control of the roots of P.japonicus var.major.关键词
珠子参/皂苷成分/薄层色谱/HPLC特征图谱Key words
The roots of Panax japonicus var.major/saponins/TLC/HPLC fingerprints引用本文复制引用
张海元,夏伟军,谢佳颖,唐梦云,张英杰,崔蓉,梅双喜,杨立国..珠子参药材中皂苷类成分的薄层色谱及HPLC特征图谱研究[J].中国现代中药,2017,19(3):358-361,414,5.基金项目
云南省科技领军人才培养计划项目(2014HA001) （2014HA001）
