中国现代中药2017，Vol.19Issue(3)：372-376,5.DOI:10.13313/j.issn.1673-4890.2017.3.013
香樟精油对金黄色葡萄球菌的抑菌活性及抑菌机理研究
Antibacterial Activity and Mechanism of Essential Oil from Cinamomum camphora on Staphylococcus aureus
摘要
Abstract
Objective:To investigate the antibacterial activity and mechanism of the essential oil from Cinamomum camphora on Staphylococcus aureus.Methods:The inhibition zone experiment and tube double dilution method were used to determine the essential oil's antimicrobial activity and the minimum inhibitory concentration(MIC).The antibacterial mechanism experiments were carried out by determining changes of reducing sugar content,protein content and electric conductivity.Results:The MIC to S.aureus was 12.5%.The essential oil could affect the membrane permeability of S.aureus.After treated with the essential oil for 4 hours,the reducing sugar content of the experimental groups was increased by 25.48%,31.02%,31.25%(P<0.05)than that of the control group,respectively,and the total content of proteins increased by 26.86%,31.43%,31.23%(P<0.05),respectively.The difference was significant.Conclusion:The essential oil showed obvious antibacterial activity against S.aureus.The antimicrobial mechanism is inferred that bacterial cell structure is damaged,cell membrane permeability increased.关键词
香樟精油/抑菌活性/抑菌机理/抑菌圈直径Key words
Essential oil/antimicrobial activities/antibacterial mechanism/inhibition zone diameters.引用本文复制引用
杨英铎,陆大洪,杨冬梅,袁小晶,秦海燕..香樟精油对金黄色葡萄球菌的抑菌活性及抑菌机理研究[J].中国现代中药,2017,19(3):372-376,5.基金项目
四川省教育厅基金项目(14zd1126) （14zd1126）
西南科技大学大学生创新基金(CX16-069) （CX16-069）
