中国现代中药2017，Vol.19Issue(3)：430-435,6.DOI:10.13313/j.issn.1673-4890.2017.3.025
不同规格商品丹参质量比较研究
Quality Comparation of Various Commercial Products of Salviae Miltiorrhizae Radix et Rhizoma
摘要
Abstract
Objective:The study is aimed to establish an HPLC method for simultaneous determination of tanshinol,protocatechuic acid,protocatechualdehyde,salvianolic acid B,salvianolic acid A,dihydrotanshinone I,tanshinone I,cryptotanshinone and tanshinone IIA in commercial products of Salviae Miltiorrhizae Radix et Rhizoma (CPSMR),and evaluate the qualities of 20 samples from different specifications of CPSMR.Methods:20 batches of S.miltiorrhiza were collected from Zhejiang,Shandong,Jiangsu,Hebei,Henan,Sichuan and other habitats.The determination was performed on a Waters xBridgeTM C18 column(250 mm×4.6 mm,5 μm)with a gradient mobile phase consisting of methanol(A)-0.03% phosphoric acid solution(B)at a flow rate of 1.0 mL·min-1.The column temperature was 25 ℃ and the detection wavelength was 270 nm.Cluster analysis was conducted with DPS software for comprehensive quality evaluation of 20 batches of CPSMR.Results:All the 9 effective constituents showed good linearity.The RSD of the precision,repeatability and stability tests were less than 3%.The average recoveries were in the range of 95.13%-101.35%.In view of habitats,CPSMR from Sichuan province were better than those from Hebei,Jiangsu and Zhejiang province.CPSMR from Henan and Shandong have the samples purchased from pharmacies.Conclusion:The contents of 9 effective constituents are different among Salviae Miltiorrhizae Radix et Rhizoma of differet specifications in markets from various habitats.SMRR products from Shandong and Henan have higher contents of tanshinones and salvianolic acids.HPLC can be used for the simultaneous determination of 9 effective constituents in SMRR products,which could be applied for the comprehensive quality evaluation of SMRR products.关键词
丹参/高效液相色谱法/多成分/含量测定/质量评价Key words
Salviae Miltiorrhizae Radix et Rhizoma/HPLC/multicomponent/content determination/quality evaluation引用本文复制引用
桑梦如,黄楚璇,吴啟南,樊修和,乐巍,吴达维,许一鸣..不同规格商品丹参质量比较研究[J].中国现代中药,2017,19(3):430-435,6.基金项目
江苏省高校优势学科建设工程资助项目(2014) （2014）
国家公益性行业专项(201407002) （201407002）
国家公共卫生行业专项(2014002) （2014002）
