中医药信息2017，Vol.34Issue(1)：1-5,5.
大黄酸对3T3-L1前脂肪细胞增殖分化及相关基因表达的影响
Effect of Rhein on 3T3-L1 Preadipocyte Differentiation and Related Gene Expression
摘要
Abstract
Objective:The effect of rhein (1,8-dihydroxy-3-carboxyanthraquinone,Rhein,Rh) on the proliferation and differentiation of 3T3-L1 adipocyte and the related gene mRNA expression was investigated.Method:MTT assay was used to detect 3T3-L1 cell proliferation by rhein (5,10,20,40,80,160 μM),rhein intervention 3T3-L1 adipocytes induced differentiation,oil red O staining and colorimetric analysis of the impact of rhein on 3T3-L1 adipocyte differentiation process of the accumulation of lipid droplets in the cytoplasm,biochemical detected rhein 3T3-L1 adipocytes triglyceride (TG).The expression of CCAAT enhancer binding proteins (C/EBPot),fatty acid synthetase (FAS),peroxisome proliferators activated receptor γ (PPARγ) genes was measured by real-time PCR.Results:The results showed that rhein (20,40,80 μM) could inhibit adipocyte differentiation and reduce intracellular TG content in a dose-dependent manner.Furthermore,rhein down-regulated the mRNA expression of C/EBPoα,FAS,and PPARγgenes.Conclusion:Rhein can inhibit the differentiation of 3T3-L1 adipocytes,and the mechanism of action may be related to reducing adipose differentiation-related gene PPARγ,C/EBPα and FAS expression.关键词
大黄酸/3T3-L1前脂肪细胞/增殖分化/基因表达Key words
Rhein/3T3-L1 preadipocytes/Proliferation and differentiation/Gene expression分类
医药卫生引用本文复制引用
李佳佳,梁耀月,董世芬,杨蓉芳,肖丛瑞,王晶..大黄酸对3T3-L1前脂肪细胞增殖分化及相关基因表达的影响[J].中医药信息,2017,34(1):1-5,5.基金项目
国家自然科学基金资助项目(No.81503287,81430094) （No.81503287,81430094）
教育部博士点基金项目(No.20130013120002) （No.20130013120002）
