中医药信息2017，Vol.34Issue(1)：6-10,5.
乌腺金丝桃与黄芪配伍对大鼠心肌缺血再灌注损伤的保护作用
Protective Effect of Compatibility of Hypericum Attenuatum and Astragalus Mongholicus on Myocardial Ischemia Reperfusion Injury
摘要
Abstract
Objective:To investigate the protective effect of compatibility of Hypericum attenuatum and Astragalus mongholicus on myocardial ischemia reperfusion injury (MIRI).Methods:Myocardial ischemia reperfusion injury models were established by ligation of the left anterior descending artery of the coronary artery.Speroxide dismutase (SOD) activity and malondialdehyde (MDA) content were determined in rats' serum.TUNEL method was used to detect the apoptosis of myocardial cells.Caspase-3 and TNF-α proteins were determined by immunohistochemistry in myocardial cells of myocardial ischemia and reperfusion.Results:Compared with the model group,the pretreatment group and the H + AM1:2 group could significantly increase SOD activity and reduce MDA content,and H + AM1:2 group could significantly reduce the myocardial apoptosis index in rats.The apoptosis index of myocardial cells was significantly reduced in the pretreatment group.Compared with sham operation group and model group,the protein amount of Caspase-3 and TNF-α in model group was significantly increased.Compared with the model group,H + AM1:2 group and pretreatment group could significantly reduce Caspase-3 and TNF-α protein amount.Conclusion:It has a protective effect of compatibility of Hypericum attenuatum and Astragalus mongholicus on myocardial ischemia reperfusion injury.关键词
乌腺金丝桃/黄芪/心肌缺血再灌注/抗氧化应激/Caspase-3/TNF-αKey words
Hypericum attenuatum/Astragalus mongholicus/Myocardial ischemia reperfusion/Anti-oxidative stress/Caspase-3/TNF-α分类
医药卫生引用本文复制引用
付殷,高彦宇,曹明明,王艳丽,李冀..乌腺金丝桃与黄芪配伍对大鼠心肌缺血再灌注损伤的保护作用[J].中医药信息,2017,34(1):6-10,5.基金项目
国家自然科学基金项目(No.813733536) （No.813733536）
