中医药信息2017，Vol.34Issue(1)：36-39,4.
HPLC-ELSD法同时测定无梗五加果中3种3,4-裂环羽扇豆烷型三萜化合物的含量
Simultaneous Determination of Three 3,4-seco-Lupane-type Triterpenoids in Acanthopanax Sessiliflorus Fruits by HPLC-ELSD
摘要
Abstract
Objective:To establish a high performance liquid chromatography-evaporative light scattering detection (HPLC-EISD) method for the simultaneous determination of 22-α-hydroxychiisanogenin (1),chiisanogenin (2),(1 R,11 α) 1,4-epoxy-11-hydroxy-3,4-secolupane-20 (30)-ene-3,28-dioic acid (3) in the Acanthopanax sessiliflorus fruits.Methods:The Diamonsil C1s column (250 mm x 4.6 mm,5 μm) was used,using gradient elution with a mobile phase of methanol as solvent A and 0.2％ (V/V) acetic acid in water as solvent B;the flow rate was 1.0 mL/min;the column temperature was maintained at 25 ℃.The ELSD conditions were as follows:the evaporative temperature was set at 60℃;the drift tube temperature was set at 45 ℃;the gas flow rate was 1.60 L/min.Results:The three compounds were completely separated within 70 min.The regression equation showed a good liner relationship between logarithm of the peak area and logarithm of the content of each compound (r =0.999 5-0.999 7).The average recoveries (n =6) were 102.9％ (RSD =1.9％),95.4％ (RSD =3.0％) and 98.7％ (RSD =2.3％),respectively.The contents of compounds 1-3 in samples were 9.37-9.61,2.03-2.18,0.39-0.45 rmg/g,respectively.Conclusion:The method is simple,fast and reliable,which can be used to determine the contents of compounds 1-3 in A.sessiliflorus fruit.关键词
无梗五加果/高效液相色谱-蒸发光散射检测/含量测定Key words
Acanthopanax sessiliflorus fruit/HPLC-ELSD/Determination分类
医药卫生引用本文复制引用
吴高松,王知斌,杨春娟,刘华,王秋红,杨炳友,匡海学..HPLC-ELSD法同时测定无梗五加果中3种3,4-裂环羽扇豆烷型三萜化合物的含量[J].中医药信息,2017,34(1):36-39,4.基金项目
国家重点基础研究发展计划-973计划(No.2013CB531801) （No.2013CB531801）
黑龙江中医药大学“优秀创新人才”支持项目(No.2012RCD05) （No.2012RCD05）
黑龙江中医药大学“杰出人才培养”基金项目(No.2012cj02) （No.2012cj02）
