组织工程与重建外科杂志2017，Vol.13Issue(2)：77-81,5.DOI:10.3969/j.issn.1673-0364.2017.02.005
藏红花素对光老化皮肤成纤维细胞的影响
Effects of Crocin on Photoaged Skin Fibroblasts
邓明武 1张溢晨 2李东 3于子优 1王祥盛 2张文杰1
作者信息
- 1. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科
- 2. 上海市组织工程重点实验室
- 3. 201108 上海市 上海星河湾双语学校
- 折叠
摘要
Abstract
Objective To investigate the protective effects of crocin on photoaged skin fibroblasts, and elucidate the mechanism of crocin on anti-photoaging, which could provide new evidence for clinical application of crocin. Methods 1. Human skin fibroblasts were isolated and treated with different concentration of crocin (0, 50, 100, 100μM) for 72 hours. CCK-8 assay was performed to detect cell proliferation. 2. Fibroblasts were irradiated with different doses of ultraviolet light (25, 50, 100 mJ/cm2), cell proliferation was measured by CCK-8 kit after 72 hours. 3. Fibroblasts were treated with different concentration of crocin (0, 50, 100, 100μM) for 24 hours and irradiated by ultraviolet light (100 mJ/cm2). Intracellular reactive oxygen species (ROS) were analyzed immediately by flow cytometry. Cell cycles were analyzed 48 hours after irradiation by flow cytometry. Seventy-two hours after treatment, cell proliferation was measured by CCK-8 kit, total RNA was extracted, and the expression of type Ⅰ collagen were detected by RT-PCR. Results 1. Low concentration of crocin (50, 100 μM) had no significant effect on the proliferation of skin fibroblasts while high concentration of crocin (200μM) promoted cell proliferation. 2. Ultraviolet irradiation inhibited the proliferation of skin fibroblast in a dose dependent manner. 3. Ultraviolet irradiation enhanced cellular level of ROS, and inhibited cell proliferation and the expression of typeⅠcollagen. Crocin (12.5, 25, 50, 100μM) treatment reduced the production of ROS caused by ultraviolet irradiation, and restored the cell cycle, cell proliferation, and type I collagen expression. Conclusion Crocin prevent the photoaging of dermal fibroblasts against ultraviolet irradiation by reducing the cellular level of ROS and restoring the cell cycle and type I collagen expression.关键词
皮肤光老化/藏红花素/抗氧化Key words
Skin photoaging/Crocin/Antioxidation分类
医药卫生引用本文复制引用
邓明武,张溢晨,李东,于子优,王祥盛,张文杰..藏红花素对光老化皮肤成纤维细胞的影响[J].组织工程与重建外科杂志,2017,13(2):77-81,5.
