安徽农业科学2017，Vol.45Issue(7)：114-115,156,3.
电刺激对铁皮石斛原球茎中石斛碱含量的影响
Effects of Electrostimulation on Dendrobine Content in Protocorm of Dendrobium officinale
摘要
Abstract
[Objective] To investigate the effects of electrostimulation on dendrobine content in protocorm of Dendrobium officinale.[Method] Protocorms were cultured by protocorm culture medium and randomly divided into two groups, the experimental group was treated with electrostimulation(25 V) while the control group did not be disposed.After 15 days incubation, dendrobine was extracted by chloroform and hot-refluxing, and determinated by ultraviolet spectrophotometry.[Result] Compared with the control group, the dendrobine content of experimental group treated with electrostimulation(25 V) for 1 h or 2 h was increased significantly(P<0.05), while the dendrobine content was decreased(P<0.05) in experimental group treated by electrostimulation(25 V) for 3 h.[Conclusion] The electrostimulation(25 V) for 1 h or 2 h can promote the accumulation of dendrobine in protocorm, and the mechanism may be related to the changed permeability of cell, the enhanced absorption of nutrients and the increased endogenous secondary metabolites.关键词
铁皮石斛/原球茎/电刺激/石斛碱/紫外分光光度法Key words
Dendrobium officinale/Protocorm/Electrostimulation/Dendrobine/Ultraviolet spectrophotometry分类
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何清英,罗志华,林棉杰,陈俊杰,邓秋怡,温斌,陈珺..电刺激对铁皮石斛原球茎中石斛碱含量的影响[J].安徽农业科学,2017,45(7):114-115,156,3.基金项目
广东药科大学大学生创新创业训练计划项目(国家级/省级,201410573006). （国家级/省级,201410573006）
