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复合酶法提取菊芋菊糖的工艺优化

江玉婷 陈靖超 李程程 刘丽萍 汪自慧 杭华

安徽农业科学2017,Vol.45Issue(8):98-102,5.
安徽农业科学2017,Vol.45Issue(8):98-102,5.

复合酶法提取菊芋菊糖的工艺优化

Optimization of Complex Enzymatic Extraction Technology of Inulin from Jerusalem artichoke

江玉婷 1陈靖超 1李程程 1刘丽萍 1汪自慧 1杭华1

作者信息

  • 1. 安徽师范大学,安徽芜湖 241002
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摘要

Abstract

[Objective] To optimize the complex enzymes extraction of inulin from Jerusalem artichoke.[Method] The effects of solid-liquid ratio, pH, temperature, enzyme contents on the yield of inulin were explored.On the basis of this, the response surface methodology was adopted to optimize operation parameters and obtain the best extraction conditions.[Result] The results showed that the optimum conditions were the radio of material-to-liquid of 1∶20 (g∶mL), pH 6.0, temperature of 54 ℃, compound enzymes(mpapain∶mpectinase=1∶8) with enzyme sample of 131 μg/g and extraction time of 40 mins.Under the optimum conditions, the inulin yield could obtain about 73.30%.[Conclusion] The study will provide the thesis basis for further industrial production.

关键词

菊芋/菊糖/复合酶法/响应面优化/提取

Key words

Jerusalem artichoke/Inulin/Compound enzymatic method/Response surface analysis/Extraction

分类

农业科技

引用本文复制引用

江玉婷,陈靖超,李程程,刘丽萍,汪自慧,杭华..复合酶法提取菊芋菊糖的工艺优化[J].安徽农业科学,2017,45(8):98-102,5.

基金项目

安徽师范大学2015年在校大学生创新创业训练计划项目(201510370025) (201510370025)

2016年安徽省教育厅自然基金重点项目(611607) (611607)

安徽师范大学"12博士科研启动项目"(161-070110). (161-070110)

安徽农业科学

0517-6611

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