安徽农业科学2017，Vol.45Issue(9)：98-100,148,4.
聚合酶链式反应技术在食品源性分析中的应用
Application of Polymerase Chain Reaction in Food Derived Materials Determination
姚艳玲1
作者信息
- 1. 嘉兴市食品药品检验检测院,浙江嘉兴 314001
- 折叠
摘要
Abstract
[Objective] The application of different polymerase chain reactions(PCRs) in food derived materials′ determination were compared,in order to analyze the diversification and prospect of the primer,dye and probe in PCR.[Method] The techniques of PCR and fluorogenic quantitative PCR were introduced,and the characters and the applications of these techniques in the determination of food derived materials were compared.[Result] The sensitivity and availability of the technology of PCR can be improved by improving extraction methods of DNA,optimizing the reaction systems,and designing the distinct primes or probes.[Conclusion] The technologies of PCR and fluorogenic quantitative PCR can be used in the determinations of food derived materials,and they have the characteristics of sensitive,efficient and specific.关键词
聚合酶链式反应/荧光定量聚合酶链式反应/源性成分Key words
Polymerase chain reaction/Fluorogenic quantitative PCR/Derived materials分类
轻工纺织引用本文复制引用
姚艳玲..聚合酶链式反应技术在食品源性分析中的应用[J].安徽农业科学,2017,45(9):98-100,148,4.
