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紫外分光光度法测定甘草渣中总黄酮含量

张腊腊 胡浩斌 王宗博 武芸 韩明虎

安徽农业科学2017,Vol.45Issue(9):123-124,131,3.
安徽农业科学2017,Vol.45Issue(9):123-124,131,3.

紫外分光光度法测定甘草渣中总黄酮含量

Determination of Total Flavonoids in Licorice Residue by UV Spectrophotometry

张腊腊 1胡浩斌 1王宗博 2武芸 1韩明虎1

作者信息

  • 1. 陇东学院化学化工学院,甘肃庆阳 745000
  • 2. 庆阳市中凯农产品有限责任公司,甘肃庆阳 745000
  • 折叠

摘要

Abstract

[Objective] The research aimed to establish the determination method of total flavonoids content in licorice residue.[Method]Using rutin as the reference substance and 10% KOH as the color reagent,the content of total flavonoids in the samples was measured at 409 nm by UV spectrophotometry.[Result]The total flavonoids showed a good linearity in a certain range,R2 was 0.994 5.The average recoveries were 88%,RSD was 1.3%,and the content of total flavonoids in licorice residue was 2.24%.[Conclusion] The method is stable and simple,and is suitable for the determination of total flavonoids in licorice residue.

关键词

紫外分光光度法/甘草渣/总黄酮含量

Key words

UV spectrophotometry/Licorice residue/Total flavonoids content

分类

农业科技

引用本文复制引用

张腊腊,胡浩斌,王宗博,武芸,韩明虎..紫外分光光度法测定甘草渣中总黄酮含量[J].安徽农业科学,2017,45(9):123-124,131,3.

基金项目

甘肃省科技支撑项目(1604NKCM082) (1604NKCM082)

甘肃省应用化学省级重点学科建设项目(GSACK20130113) (GSACK20130113)

陇东学院青年基金项目(XYZK1614). (XYZK1614)

安徽农业科学

0517-6611

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