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多粘类芽孢杆菌DN-1固态发酵条件优化

胡飞 苏卫华 胡本进 周子燕 徐丽娜 陈浩梁 苏贤岩

安徽农业科学2017,Vol.45Issue(9):151-154,4.
安徽农业科学2017,Vol.45Issue(9):151-154,4.

多粘类芽孢杆菌DN-1固态发酵条件优化

Optimizing Solid Fermentation Condition for Paenibacillus polymyxa DN-1

胡飞 1苏卫华 1胡本进 1周子燕 1徐丽娜 1陈浩梁 1苏贤岩1

作者信息

  • 1. 安徽省农业科学院植物保护与农产品质量安全研究所,安徽合肥 230031
  • 折叠

摘要

Abstract

[Objective] The aim was to discuss solid fermentation condition for Paenibacillus polymyxa DN-1.[Method] We optimized the best solid medium and fermentation conditions of Paenibacillus polymyxa DN-1 by the single factor and muti factor experiments.[Result] The optimum medium for Paenibacillus polymyxa DN-1 was 15% rice husk,40% corn flour,20% potato,10% oil-tea-cake,15%water,0.1% K2HPO4 and 0.2% MnSO4.The optimal fermentation conditions were fermentation time for 72 h,temperature at 37 ℃,pH at 6.5-7.0 and 10% inoculation quantity.The yield and ratio of spore formation were 2.63 billion spores /g and 92%,respectively.[Conclusion] The results provide reference for further study and application of Paenibacillus polymyxa DN-1.

关键词

多粘类芽孢杆菌DN-1/活菌数/芽孢率/发酵/优化

Key words

Paenibacillus polymyxa DN-1/Yield/Ratio of spore formation/Fermentation/Optimize

分类

农业科技

引用本文复制引用

胡飞,苏卫华,胡本进,周子燕,徐丽娜,陈浩梁,苏贤岩..多粘类芽孢杆菌DN-1固态发酵条件优化[J].安徽农业科学,2017,45(9):151-154,4.

基金项目

安徽省农业科学院院长青年创新基金项目(16B1121) (16B1121)

安徽稻麦(油)轮作区水稻化肥农药减施增效技术集成与示范(2016YFD0200806). (油)

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