安徽医科大学学报2017，Vol.52Issue(4)：542-545,4.DOI:10.19405/j.cnki.issn1000-1492.2017.04.017
miR-105-1在原发性肝细胞癌中表达的研究
Down-regulated miR-105-1associated with hepatocellular carcinoma patients
摘要
Abstract
Objective To explore the expression level of miR-105-1 in hepatocellular carcinoma(HCC).Methods Total RNA was extracted from 10 pairs Tumor-Non-tumor(T-N) pairing tissue samples and another 40 HCC tissue samples, real-time quantitative PCR was performed.The 2-ΔΔCt method was used to quantify the expression level of miR-105-1.The gene chip data of HCC obtained from the GEO database were used to analyze the expression level of miR-105-1 in liver cancer.Results The results showed that the expression of HCC tissues of miR-105-1 was significantly lower than adjacent normal tissues in the 10 T-N paired tissue samples, the difference was statistically significant between the two(P=0.046).Equally, the expression of HCC tissues of miR-105-1 was significantly lower in the 50 HCC tissue samples than that in 10 adjacent normal tissues, the difference was statistically significant(P=0.031).The gene chip from GEO database(GSE22058-GPL10457) including 96 T-N paired HCC tissue samples suggested that miR-105-1 was down-regulated(P=0.035).Another data(GSE21362) including 73 T-N paired HCC tissue samples indicated that miR-105-1 was also down-regulated (P=0.042).Conclusion miR-105-1 is an important molecule in the progress of HCC patients.The expression level of HCC patients is lower than healthy people.It might be used as a prognostic indicator and a new target for HCC therapy.关键词
miR-105-1/肝细胞癌/表达Key words
miR-105-1/hepatocellular carcinoma/expression分类
医药卫生引用本文复制引用
吴庭苗,马雨水,卢改霞,秦珊珊,赵腊梅,吕中伟..miR-105-1在原发性肝细胞癌中表达的研究[J].安徽医科大学学报,2017,52(4):542-545,4.基金项目
国家自然科学基金(编号:81371595) （编号:81371595）
