安徽医科大学学报2017，Vol.52Issue(4)：597-601,5.DOI:10.19405/j.cnki.issn1000-1492.2017.04.030
c-met慢病毒载体的构建及稳定转染骨髓间充质干细胞
Construction of c-met lentiviral vector and stable transfected bone marrow mesenchymal stem cells
摘要
Abstract
To construct cellular-mesenchymal to epithelial transition factor(c-met) lentiviral vector, then transfect it into bone marrow mesenchymal stem cells(BMSCs) stably.c-met was connected to the lentiviral vector GV358, and the lentiviral vector GV358-c-met was restructed.Then the recombinant and lentiviral helper plasmid were co-transfected into 293T cells to generate the traget lentivirus which the titer was 2×108 TU/ml.After transfecting this lentiviral vector into BMSCs and screening by puromycin, the positive rate of fluorescence was 100% by fluorescence microscopy, and the expression of c-met protein was confirmed by Western blot.Therefore, we successfully constructed c-met lentiviral vector, and established the BMSCs with the characteristics of stable expression c-met protein.关键词
骨髓间充质干细胞/c-met/慢病毒载体Key words
bone marrow mesenchymal stem cell/c-met/lentiviral vector分类
医药卫生引用本文复制引用
王坤,朱甜甜,李毓雯,李军,朱传龙..c-met慢病毒载体的构建及稳定转染骨髓间充质干细胞[J].安徽医科大学学报,2017,52(4):597-601,5.基金项目
国家自然科学基金(编号:81271713) （编号:81271713）
吉利德肝病科学研究项目-亚洲 （）
