北华大学学报（自然科学版）2017，Vol.18Issue(3)：321-325,5.DOI:10.11713/j.issn.1009-4822.2017.03.009
五味子淫羊藿混合物提取工艺及其抗疲劳作用研究
Optimal Extraction Process and Antifatigue of Mixed Extract of Schisandra chinensis and Epimedium sagittatum
摘要
Abstract
Objective To study the optimal extraction process of the mixed extract of Schisandra chinensis and Epimedium sagittatum,and the antifatigue of the extract in vitro. Method The mixture of Schisandra chinensis and Epimedium sagittatum was extracted with heating reflux extraction. Orthogonal experiment effects of the ethanol concentration (65%,70% and 75%),the extraction temperature (60,65 and 75℃),the extraction time (1,1. 5 and 2 h) and the solid-liquid ratio (1 : 10,1 : 14 and 1 : 18) on the extraction process were observed, and the Schisandrin and Icariine contents were analyzed respectively,through which the extraction process of the mixed extract of Schisandra chinensis and Epimedium sagittatum was optimized. 80 male ICR mice were selected and randomly divided into control group,and 150,300 and 600 mg/kg MSEE groups,20 rats in each group. After the administration for 30 days,10 mice randomly selected from each group were for exhaustive swimming test,and the exhaustive swimming time of mice was recorded. The remaining 10 mice in each group were sacrificed after the swimming for 90 minutes,and their blood and liver tissue samples were taken for the detection of serum urea nitrogen and liver glycogen levels. Results The optimal extraction process of the mixed extract of Schisandrachinensis and Epimedium sagittatum was as follows: the ethanol concentration was 70%, the extraction temperature was 75℃,the extraction time was 1.5 h and the solid-liquid ratio was 1:14. Compared with those in the control group,swimming times of mice in 300 and 600 mg/ kg MSEE groups were significantly prolonged (P<0.01);serum urea nitrogen levels of mice in 300 and 600 mg/ kg MSEE groups were significantly lowered,and liver glycogen levels were significantly elevated (P<0.05 or P<0.01). Conclusion The optimal extraction process of MSEE was confirmed in this study. MSEE has an antifatigue activity. This study may provide the basis for the development of health food with antifatigue function.关键词
五味子/淫羊藿/混合提取/抗疲劳Key words
Schisandra chinensis/Epimedium sagittatum/mixed extraction/antifatigue分类
化学化工引用本文复制引用
王晗,刘聪,敬舒,蒋维海,孙靖辉,王春梅,陈建光,李贺..五味子淫羊藿混合物提取工艺及其抗疲劳作用研究[J].北华大学学报（自然科学版）,2017,18(3):321-325,5.基金项目
吉林省科技厅科技发展计划项目(20140204012YY （）
20150312041ZG) （）
吉林市科技发展计划项目(20166018 （）
20163054). （）
