北华大学学报（自然科学版）2017，Vol.18Issue(3)：345-347,3.DOI:10.11713/j.issn.1009-4822.2017.03.014
不同制备工艺所得毛樱桃总黄酮的抗炎活性研究
Preparation Technology of Total Flavonoids Prunus tomentosa Optimization and Anti-Inflammatory Activity Screening
摘要
Abstract
Objective To study the optimum extraction technology of total flavonoids from Prunus tomentosa,and determine the content of total flavonoids in the extracts,in order to provide theoretical basis for the further study of the manchu cherry. Method The system solvent extraction method was used to enrich and purify total flavonoids in the ethanol extracts. The anti-inflammatory activity of the extracts was investigated by mouse ear swelling test. Results The optimized extraction and purification process could obviously improve the extraction rate of total flavonoids;The content of total flavonoids in water and petroleum ether extract was 1 . 31% and 5. 59%,respectively,and the content of total flavonoids in ethyl acetate extract was 26. 31%;The effects of the three extracts on the degree of swelling ( mg ) of the ear swelling model mice were 11 . 03 ± 3 . 44 ( water extract),11. 29 ± 2. 92 (petroleum ether extract) and 6. 56 ± 2. 59 (ethyl acetate extract),respectively. Conclusion The total flavonoids of Prunus tomentosa can be extracted and enriched by ethanol, petroleum ether,water and ethyl acetate. The yield, purity and anti-inflammatory activity of the total flavonoids can be improved by the optimized technology significantly.关键词
毛樱桃/总黄酮/系统溶剂提取法/抗炎Key words
Prunus tomentosa Thunb' s/total flavonoids/solvent extraction method/anti-inflammation分类
医药卫生引用本文复制引用
师继超,赵东双,刘圆圆,王曼力,赵楠晰,张成义..不同制备工艺所得毛樱桃总黄酮的抗炎活性研究[J].北华大学学报（自然科学版）,2017,18(3):345-347,3.基金项目
吉林省科技发展计划项目(20150311024YY) （20150311024YY）
吉林省卫生技术创新项目(2016J087). （2016J087）
