北京大学学报（医学版）2017，Vol.49Issue(2)：337-343,7.DOI:10.3969/j.issn.1671-167X.2017.02.027
低能量激光照射对人脂肪基质细胞增殖分化的影响
Effect of low-level laser irradiation on proliferation and osteogenic differentiation of human adipose-derived stromal cells
摘要
Abstract
Objective:To examine the in vitro effects of low-level laser irradiation (LLLI) on proliferation and differentiation of human adipose-derived stromal cells(hASCs).Methods: Cultured cells were exposed to different doses of LLLI with a semiconductor diode laser (980 nm;100 mW-12 W power output).The effects of laser on proliferation were assessed daily up to seven days of culture in cells irradiated for four consecutive days with laser doses of 2,4,6 or 8 J/cm2,the cells without irradiation were used as controls.Half of the cells were changed to osteogenic medium (OM) when they had grown to 70％ confluence.The hASCs both with and without osteogenic supplements were divided into three groups,and each group was irradiated at doses of 0,2 and 4 J/cm2.In order to examine the in vitro effects of LLLI on osteogenic differentiation of hASCs,the alkaline phosphatase activity was assessed on day 7,and alizarin red staining (AR-S) and quantitative detection were assessed on days 14 and 21.The expression of osteoblast master genes (ALP and Runx2) were tested on days 7 and 14.Results: The proliferation medium(PM)+LLLI4 J/cm2 group had the highest multiplication rate.In the groups with osteogenic supplements,LLLI increased alkaline phosphatase activity and mineralized nodule formation,and stimulated the expression of ALP and Runx2.Furthermore,the effect became more obvious at high dose.Conclusion: Our data demonstrated that hASCs proliferation and osteogenic differentiation were enhanced by LLLI.With the increase of laser dose,the effect of LLLI would be enhanced at first,and then be decreased after reaching a peak.关键词
低能量激光照射/人脂肪基质细胞/细胞增殖/细胞分化/组织工程Key words
Low-level laser irradiation/Adipose tissue human adipose-derived stromal cells/Cell proliferation/Cell differentiation/Tissue engineering分类
医药卫生引用本文复制引用
隋华欣,吕培军,王宇光,王勇,孙玉春..低能量激光照射对人脂肪基质细胞增殖分化的影响[J].北京大学学报（医学版）,2017,49(2):337-343,7.基金项目
教育部科学技术研究项目(113002A)资助Supported by Project of Chinese Ministry of Education,China (113002A) （113002A）
