北京中医药大学学报2017，Vol.40Issue(4)：297-301,5.DOI:10.3969/j.issn.1006-2157.2017.04.007
脾虚四号方含药血清对功能性腹泻脾虚证大鼠离体结肠平滑肌细胞的影响
In vitro effects of Pixu Sihao Fang dosed serum on colonic smooth muscle cells in rats with spleen-deficiency functional diarrhea
摘要
Abstract
Objective To study the cellular changes of colonic smooth muscle cells in rats with spleen-deficiency functional diarrhea treated with Pixu Sihao Fang (PXSHF, No.4 Formula for Spleen Deficiency) dosed serum.Methods Colonic smooth muscle cells from functional diarrhea spleen-deficiency rats were extracted for primary culture and then divided into the following six groups: normal control group, model group, PXSHF low-dose, medium-dose, and high-dose groups, and montmorillonite powder (Mnt) group.All the dosed serum groups were treated with 5% drug serum for 48 h.CCK-8 test for detecting the cell proliferation activity and QRT-PCR test for checking the expression of brain-gut petide mRNA were performed.Results OD value of the model group was significantly lower than that of the control group (P<0.01) in CCK-8 test while the OD value of the drug serum groups were significantly higher than that of the model group (P<0.01).QRT-PCR test showed that the CCK/VIP/SS mRNA expression of the model group was significant lower than that of the control group (P<0.05).Compared with the model group, the CCK/VIP/SS mRNA expression in each drug serum group presented with an increase tendency.CCK mRNA expression of the group treated with low-dose PXSHF dosed serum was higher than of the model group (P<0.05).VIP mRNA expression of the groups treated with PXSHF dosed serum were significantly higher than that of the model group (P<0.01).Conclusion PXSHF dosed serum can effectively promote the proliferation activity of the colonic smooth muscle cells.Changes in the expression of CCK, VIP and SS mRNA might be indicative of the role of brain-gut peptide in the development of functional diarrhea with spleen deficiency pattern.关键词
脾虚四号方/功能性腹泻/脾虚证/脑肠肽/大鼠Key words
PXSHF dosed serum/functional diarrhea/spleen deficiency/brain-gut petide/Rats分类
医药卫生引用本文复制引用
陈旻丹,林炳岐,陈斗,李峰,胡京红,马捷,吴凤芝,刘燕,唐旭东,王凤云,韩晨霞,戴宁..脾虚四号方含药血清对功能性腹泻脾虚证大鼠离体结肠平滑肌细胞的影响[J].北京中医药大学学报,2017,40(4):297-301,5.基金项目
国家重点基础研究发展计划(973计划)项目(No.2013CB531703),北京中医药大学在读研究生项目(No.2016-JYB-XS004) （973计划）
