北京中医药大学学报2017，Vol.40Issue(4)：302-307,327,7.DOI:10.3969/j.issn.1006-2157.2017.04.008
逍生散对干眼杯状细胞影响的实验研究
Effects of Xiaosheng San on goblet cells of dry eyes in mice: a laboratory study
摘要
Abstract
Objective To study the effects of Xiaosheng San(XSS, Xiaosheng Granules) on goblet cells of dry eyes in mice and evaluate its mechanism.Methods 180 male C57BL/6 mice were divided into six groups: normal control (group A), model (group B), saline (group C), XSS low-dose (group D), XSS medium-dose (group E) and high-dose (group F) groups.Dry eye model was established by injecting scopolamine subcutaneously at the base of rear legs in all groups except normal control group.Schirmer I test (SIT), tear break-up time (BUT),corneal fluorescent (FL) staining, and the number of conjunctival goblet cells before and after modeling, and at four weeks after intervention were measured.Results BUT, SIT,and number of goblet cells in group B, C, D, E,and F were significantly decreased after scopolamine treatment(P<0.05), while no significant difference (P>0.05) was found in group A.FL were significantly increased after model establishment (P<0.05).Medium-and high-dose XSS significantly improved SIT, BUT and the number of goblet cells in group E and F (P<0.05).No such changes were observed in group A, B and C (P>0.05).Conclusion Subcutaneous injecfion of scopolamine could effectively establish dry eye model in mice.Xiaosheng Granules could increase tear secretion, prolong tear break-up time, improve stability, inhibit apoptosis of goblet cells and reduce injury to eye surface.关键词
逍生散/干眼/杯状细胞/小鼠Key words
xiaoshengsan/dry eye/goblet cells/mice分类
医药卫生引用本文复制引用
祁怡馨,谢立科,郝晓凤,张志芳,侯乐,肖文峥,孙会兰,明镜..逍生散对干眼杯状细胞影响的实验研究[J].北京中医药大学学报,2017,40(4):302-307,327,7.基金项目
国家自然科学基金资助项目(No.81273805),北京市科学技术委员会项目(No.Z141100002214001) （No.81273805）
