北京中医药大学学报2017，Vol.40Issue(4)：308-313,6.DOI:10.3969/j.issn.1006-2157.2017.04.009
含红芪、黄芪玉屏风散对SAMP8小鼠脾淋巴细胞活化影响
Effects of Yupingfeng San containing Radix Hedysari or Radix Astragali on the splenic lymphocyte activation in SAMP8 mice
摘要
Abstract
Objective To compare the effects of Yupingfeng San (YPFS, Jade Wind-Barrier Powder) containing Radix Hedysari (sweetvetch root, hongqi) or Radix Astragali (astragalus root, huangqi) on the splenic lymphocyte activation in SAMP8 mice.Methods In vitro experiment: Yupingfeng San containing Radix Hedysari or Radix Astragali and saline of the same volume were given to young mice respectively via intragastric administration for 14 days to prepare dosed serum for co-culture with SAMP8 mice spleen lymphocytes;In vivo experiment: Yupingfeng San containing Radix Hedysari or Radix Astragali, thymus peptide solution and saline of the same volume were given to SAMP8 mice respectively for 14 days before their blood and spleen samples were taken for preparing dosed serum and splenic lymphocyte suspension.Levels of TNF-α in vitro and in vivo were measured with ELISA;expression of CD28 mRNA and p38MAPK mRNA in vitro and in vivo were measured by using real-time PCR and the expression of CD28 and p38MAPK by using Western blot.Results Compared with blank serum group and normal saline group, in vitro and in vivo levels of TNF-α were increased in dosed serum groups.So were the expression of splenic lymphocyte CD28, p38MAPK mRNA and protein expression.In vitro levels of TNF-α and CD28, p38MAPK mRNA expression in the hedysari group were higher than those in the astragali group (P<0.01);in vivo levels of TNF-α and CD28, p38MAPK mRNA expression in the astragali group were higher than those in the hedysari group (P<0.05).CD28 and p38MAPK mRNA expression in the hedysari group were higher than that in the astragali group (P<0.05).Changes of TCM groups were inferior to that of the thymosin group (P<0.05).Conclusion Yupingfeng San containing Radix Hedysari or Radix Astragali both played a potential role in the splenic lymphocyte activation of SAMP8 mice, which serve as a certain resistance to immunosenescence.关键词
玉屏风散/红芪/黄芪/脾淋巴细胞/免疫老化/小鼠Key words
Yupingfeng powder/Radix Hedysari/Radix Astragali/splenic lymphocyte/immunosenescence/mice分类
医药卫生引用本文复制引用
孙美花,李纬,张文君,周黔香,程卫东..含红芪、黄芪玉屏风散对SAMP8小鼠脾淋巴细胞活化影响[J].北京中医药大学学报,2017,40(4):308-313,6.基金项目
国家自然科学基金面上项目(No.81373806),中央高校基本科研业务费专项资金项目(No.223000-862107),甘肃省中医药科学技术研究课题(No.GZK-2012-43) （No.81373806）
