传感技术学报2017，Vol.30Issue(3)：373-377,5.DOI:10.3969/j.issn.1004-1699.2017.03.007
面向水产病原菌微流控检测的磁控分离方法
Magnetic Isolating Method for Aquaculture Pathogens Detection System
摘要
Abstract
Pathogenic bacteria in aquatic environment is the major cause of aquaculture diseases.However conventional methods require a long time and a great amount of cost for other reagents,and suffer from difficult isolation and lower automation.A magnetic isolating method for aquaculture pathogens detection system was proposed.A dedicated magnetic controller with magnetic capture and isolation was designed,and the magnetic isolation experiment platform based on microfluidic chip was built up.The optimum magnetic pole current,switching frequency and capture time of the platform were determined,and illustrated by the case of Escherichia coli O157:H7 the performance of the method was experimentally verified.The research results showed that the capture efficiency of magnetic isolating method reached as high as 92%.And compared with passive isolation by barriers,the capture efficiency increased by 30%,and the separating operation was flexible and controllable with higher automation,which realized pathogens efficient separation and benefitted rapid detection and early warning on aquaculture disease.关键词
水产病原菌检测/微流控芯片/磁控分离/捕获率Key words
aquaculture pathogens detection/microfluidic chip/magnetic isolation/capture efficiency分类
农业科技引用本文复制引用
郭建江,张荣标,杨宁..面向水产病原菌微流控检测的磁控分离方法[J].传感技术学报,2017,30(3):373-377,5.基金项目
中国博士后科学基金资助项目(2014M560404) （2014M560404）
江苏省农业自主创新计划项目(CX(14)2092) （CX(14）
江苏省普通高校研究生科研创新计划资助项目(CXLX12_0662) （CXLX12_0662）
常州市科技支撑计划(社会发展)项目(CE20155054) （社会发展）
国家自然科学基金项目(61673195) （61673195）
国家级大学生创新训练项目(201611055004) （201611055004）
