重庆大学学报2017，Vol.40Issue(3)：34-39,6.DOI:10.11835/j.issn.1000-582X.2017.03.004
多肽序列优化降低c-Src蛋白靶向药物的毒性风险
Reducing the toxicity of c-Src targeting drugs by optimizing amino acid sequences
摘要
Abstract
It is important to avoid promiscuous binding between an anticancer peptide and multiple proteins with SH3 domain,so as to minimize the risks of unpredictable toxic and side effects.In the present study,we applied bioinformatics methods to optimize amino acid sequences,in order to reduce the probability of promiscuous binding.Relevant peptide databases and bioinformatics tools were utilized to summarize the rules of promiscuous binding between peptides and SH3 domains.Based on that,we specifically optimized the amino acid sequences of the peptides.The results suggest that most of the modified amino acid sequences exhibit lower level of binding promiscuity,which significantly improves the overall binding specificity and reduces safety risks (P ＜ 0.05).This study provides a reference for designing targeted peptide drugs with high binding specificity and low toxicity.关键词
c-Src蛋白/SH3结构域/多肽/混杂性结合/药物毒副作用/氨基酸序列Key words
c-Src protein/SH3 domain/peptide/promiscuous binding/toxic and side effect/amino acid sequence分类
医药卫生引用本文复制引用
任彦荣,王强,李晓波..多肽序列优化降低c-Src蛋白靶向药物的毒性风险[J].重庆大学学报,2017,40(3):34-39,6.基金项目
重庆市科委前沿与应用基础研究计划项目(cstc2014jcyjA10104) （cstc2014jcyjA10104）
重庆第二师范学院校级科研创新团队(KYC-cxtd03-2017004) （KYC-cxtd03-2017004）
第二批重庆市高等学校青年骨干教师资助计划.Supported by Front and Applied Fundamental Research Plan of Chongqing Science & Technology Commission (cstc2014jcyjA10104),Program for Innovative Research Team in Chongqing University of Education (KYC-cxtd03-2017004) and the Second Round of Funding for the Young Key Teachers of University in Chongqing. （cstc2014jcyjA10104）
