重庆医学2017，Vol.46Issue(12)：1585-1587,3.DOI:10.3969/j.issn.1671-8348.2017.12.001
骨髓间充质干细胞移植对矽肺大鼠肺纤维化的作用
Effect of bone marrow mesenchymal stem cell transplantation on silicosis fibrosis in rats
摘要
Abstract
Objective To study the effects of bone mesenchymal stem cells(BMSCs)transplantation on pulmonary tissue fibrosis in silicosis rats.Methods Forty-two healthy female SD rats were randomly divided into 4 groups:group A,B,C and D.The group A,B and C received intratracheal injection of silica crystals(SiO2)to establish silicosis mouse models,while the group D received the infusion of physiological saline.BMSCs were injected by the tail vein at 6 h after modeling or on 42 d in the group B and C.Six rats in each of group A,B,and D were sacrificed on 14 d,and other 6 rats in each group were sacrificed on 56 day.The morphological changes of the lung tissues were observed by HE staining;the content of hydroxyproline(Hyp)in the lung tissue homogenate was detected by ELISA.Results The lung tissue pathological changes in the group A were dominated by acute inflammatory response on 14 d after model construction,and fibrosis reaction on 56 d;the pathological changes in the group B and C were lighter than those in the group A;while no obvious abnormality was found in the group D.The Hyp level of pulmonary tissue in the group A,B and C was significantly increased compared with the group D(P=0.000);which on 14 d after BMSCs treatment in the group B and C was significantly decreased compared with the group A(P=0.000,P=0.035),and which on 56 d after treatment in the group B was still significantly decreased(P=0.016).Conclusion No matter in the pathological stages of pulmonary alveolitis and fibrosis,BMSCs transplantation may postpone the pathological process of pulmonary fibrosis in silicosis rats.关键词
矽肺/肺纤维化/间充质干细胞/羟脯氨酸/大鼠,Sprague-DawleyKey words
silicosis/pulmonary fibrosis/mesenchymal stem cells/hydroxyproline/rats/Sprague-Dawley分类
医药卫生引用本文复制引用
陈志军,黄惠霞,谢英,李冬红,李智民,张健杰..骨髓间充质干细胞移植对矽肺大鼠肺纤维化的作用[J].重庆医学,2017,46(12):1585-1587,3.基金项目
国家职业病临床重点专科建设项目(WY2011873) （WY2011873）
深圳市科技计划项目(JCYJ20140414110951762). （JCYJ20140414110951762）
