重庆医学2017，Vol.46Issue(12)：1588-1591,4.DOI:10.3969/j.issn.1671-8348.2017.12.002
β-catenin在兔颈总动脉套管导致的动脉粥样硬化血管壁中高表达
β-catenin high expression in atherosclerotic vessel wall caused by rabbit carotid artery cannula
摘要
Abstract
Objective To investigate the effects of rabbit carotid artery cannula on atherosclerosis formation and β-catenin expression.Methods Fifteen 2-month-old New Zealand rabbits weighing(2.0±0.2)kg were randomly divided into three groups,high-fat diet group,left common carotid artery cannula group and left common carotid artery cannula plus high-fat diet group,5 cases in each group,and taking the right blood vessels in the left common carotid artery cannula group served as the control group.The animals were sacrificed after 9-week feeding,and the total rabbit carotid artery in each group was taken;the real-time PCR and immunohistochemistry were used to detect β-catenin mRNA and protein expression targeting in rabbit common carotid artery tissue.Results The real-time PCR results showed that the β-catenin mRNA expression in the left common carotid artery cannula group was higher than that in the control group,high fat diet group and the left common carotid artery cannula + high-fat diet group.The immunohistochemistry results showed that,except for the control group,various groups had the β-catenin protein location in the cytoplasma,moreover which in the left carotid artery cannula group and left common carotid artery cannula +high-fat diet group mainly located in the area of intimal hyperplasia.Conclusion β-catenin is highly expressed in the atherosclerotic vessel wall caused by rabbit carotid artery cannula.关键词
颈总动脉套管/动脉粥样硬化/β-catenin/动物实验/兔Key words
carotid artery cannula/atherosclerosis/β-catenin/animal experimentation/rabbits分类
医药卫生引用本文复制引用
刘月华,生燕,涂应杰,杨兴,欧刚卫,生欣..β-catenin在兔颈总动脉套管导致的动脉粥样硬化血管壁中高表达[J].重庆医学,2017,46(12):1588-1591,4.基金项目
国家自然科学基金资助项目(31360278) （31360278）
贵州省科技厅联合基金项目[黔科合J字LKZ(2013)28号] （2013）
遵义医学院博士启动基金(F566). （F566）
