分析测试学报2017，Vol.36Issue(3)：312-318,7.DOI:10.3969/j.issn.1004-4957.2017.03.003
基于UPLC-Q-TOF-MS法分析凉山虫草化学成分
Analysis of Chemical Constituents in Cordyceps liangshanensis by UPLC-Q-TOF-MS
摘要
Abstract
A UPLC-Q-TOF-MS method was developed for the analysis and identification of main chemical constituents in the extract of Cordyceps liangshanensis,by an Agilent SB-C18 (50 mm × 4.6rm,1.8 μm) column with gradient elution using of 0.1％ formic acid in water and acetonitrile as mobile phase at a flow rate of 0.25 mL/min.The column temperature was set at 30 2.MS analysis was based on information associated mode (IDA) with positive and negative ions collection.By using feature of PeakView such as Formula Finder,matching the mass spectrometry data of each chromatographic peak in the database and the cleavage law of each peak's level two fragment,total of 27 compounds in Cordyceps liangshanensis were identified,in which 8 compounds in Cordyceps substance had never been reported before.The main chemical constituents include alkaloids,glycosides,nucleosides,amino acids,etc.The method is accurate,reliable and efficient,and is suitable for the rapid identification of ingredients in Cordyceps liangshanensis.It provides a reference for the development and utilization of Cordyceps liangshanensis and clarification of its efficacy and material basis.关键词
凉山虫草/UPLC-Q-TOF-MS/裂解规律/化学成分Key words
Cordyceps liangshanensis/UPLC-Q-TOF-MS/cleavage law/chemical constituents分类
化学化工引用本文复制引用
秦伟瀚,刘翔,阳勇,张小梅,郭延垒,刘飞..基于UPLC-Q-TOF-MS法分析凉山虫草化学成分[J].分析测试学报,2017,36(3):312-318,7.基金项目
国家自然科学基金资助项目(30901964,81072997) （30901964,81072997）
国家重大新药创制重大新药专项项目(2013ZX0101108) （2013ZX0101108）
