分析测试学报2017，Vol.36Issue(3)：388-392,5.DOI:10.3969/j.issn.1004-4957.2017.03.016
加压毛细管电色谱分离检测虎杖根中蒽醌类成分
Determination of the Main Anthraquinones in Root of Polygonum Cuspidatum by Pressurized Capillary Electrochromatography
摘要
Abstract
A pressurized capillary electrochromatographic (pCEC) method was developed for the determination of the main anthraquinones,including rhein,emodin,aloe-emodin,chrysophanol and physcion in root of Polygonum cuspidatum.The separation was performed on a reversed-phase EP-100-20/45-3-C1s column(total length of 45 em,effective length of 20 cm,diameter of 100 μm,ODS packing inside for 3 μm).The mobile phase was composed of 20 mmol/L NaH2PO4(pH 4.7)-acetonitrile (15 ∶ 85) at a flow rate of 0.04 mL/min.Under the optimum conditions including running voltage of + 5 kV,UV detection wavelength of 254 nm,the limits of detection (S/N=3) were in the range of 0.60-2.54 μg/mL for rhein,aloe-emodin,emodin,chrysophanol and physcion,respectively,and the linear detection ranges were 3.57-162.68 μg/mL with correlation coefficients not less than 0.998 2.The established method was used in the analysis of anthraquinones in polygonum cuspidatum with good results.The recoveries at three spiked levels ranged from 91.1％ to 101.2％,with relative standard deviations (RSD) of 0.03％-3.6％.关键词
加压毛细管电色谱/虎杖/蒽醌Key words
pressurized capillary electrochromatography/polygonum cuspidatum/anthraquinone分类
化学化工引用本文复制引用
肖晓茹,冯军,程昊,黄文艺,李彦青,赵彦勇,李利军..加压毛细管电色谱分离检测虎杖根中蒽醌类成分[J].分析测试学报,2017,36(3):388-392,5.基金项目
广西自然科学基金项目资助(2014GXNSFAA118402) （2014GXNSFAA118402）
广西高等学校高水平创新团队及卓越学者计划资助 （）
