分析化学2017，Vol.45Issue(4)：529-536,8.DOI:10.11895/j.issn.0253-3820.160808
超高效液相色谱-串联质谱法同时测定茶叶中11种植物生长调节剂及吡虫啉、啶虫脒的残留
Simultaneous Determination of Plant Growth Regulators and Imidacloprid, Acetamiprid in Tea by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An efficient method for the analysis of multiclass plant growth regulators and pesticide (imidacloprid, acetamiprid) residues in tea was developed based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted with acetonitrile/formic acid (99∶1, V/V) solution, cleaned up with four sorbents including C18, strong anion exchanger (SAX), primary secondary amine (PSA) and anhydrous MgSO4. The compounds were separated on a HSS T3 column under positive/negative electrospray ionization mode, detected by scheduled multiple reaction monitoring (SMRM), and quantified by matrix-matched external standard curves. All pesticide residues showed good linearity in the concentration range of 1-200 μg/L (6-benzylaminopurine, paclobutrazol, uniconazole, forchlorfenuron, mepiquat chloride, imidacloprid, acetamiprid) or 5-1000 μg/L (2,4-dichlor-ophenoxyacetic acid, 4-chlorophenoxyacetic acid, indole-3-acetic acid, gibberellic acid, 1-naphthaleneacetic acid, indole-3-butyric acid) , with correlation coefficient (R2≥0.99). Limits of detection (LOD, S/N=3) and limits of quantitation (LOQ, S/N=10) were 0.18-9.68 μg/kg and 0.61-32.26 μg/kg, respectively. In addition, the spiked recoveries of tea samples were 73.1％-108.9％, and RSDs were 0.6％-8.0％. This method was applied to commercial samples, and all the detections were confirmed by acquiring transitions for each pesticide in the samples.关键词
茶叶/植物生长调节剂/吡虫啉/啶虫脒/超高效液相色谱-串联质谱/分散固相萃取Key words
Tea/Plant growth regulator/Imidacloprid/Acetamiprid/Ultra performance liquid chromatography-tandem mass spectrometry/Dispersive solid phase extraction引用本文复制引用
诸力,王晨,陈红平,张颖彬,周苏娟,王国庆,刘新..超高效液相色谱-串联质谱法同时测定茶叶中11种植物生长调节剂及吡虫啉、啶虫脒的残留[J].分析化学,2017,45(4):529-536,8.基金项目
本文系国家茶叶质量安全风险评估重大专项(No.GJFP2016005)、国家茶叶产业技术体系(No.CARS-23)资助 （No.GJFP2016005）
