国际口腔医学杂志2017，Vol.44Issue(3)：294-300,7.DOI:10.7518/gjkq.2017.03.009
基于高通量测序技术研究慢性牙周炎患者龈下刮治和根面平整术治疗前后龈下菌群的变化
Microbiological community analysis by high-throughput sequencing of subgingival plaque in patients with chronic periodontitis before and after subgingival scaling and root planing
摘要
Abstract
Objective This study aims to observe the changes in the microbiological community of a subgingival plaque in chronic periodondotis after subgingival scaling and root planing(SRP). The observation was performed via high-throughput sequencing. The feasibility of this approach in diagnosis and prognosis in chronic periodontitis was then explored by microflora transition. Methods Ten patients with chronic periodontitis under treatment in the Periodontics Department of Beijing Stomatological Hospital were selected in this study. From each patient, we obtained genomic DNA from the subgingival plaque before SRP and then three months after SRP in the same sampling site. High-throughput sequencing analysis was then performed to investigate the diversity and relative abundance of the subgingival plaque microflora from phyla to species.Results In the phylum level, 16 phyla were detected, of which eight constitute 99% of the sequences in the subgingival plaque samples from each time point. In the genus level, 128 different genera were detected in all time points. The results showed that the relative abundance of Tannerella decreased three months after SRP(P<0.05), whereas those of Leptotrichia and Streptococcus increased three months after SRP(P<0.05). In the species level, 6 putative pathogens were detected. The relative abundance of Tannerella forsythia and Prevotella intermedia decreased three months after SRP(P<0.05). Conclusion The microflora of a subgingival plaque in chronic periodondotis is diverse, and the relative abundance of pathogens decreased after SRP, whereas the probiotics increased. Thus, SRP can change the microbiological community.关键词
慢性牙周炎/龈下刮治和根面平整术/高通量测序Key words
chronic periodontitis/subgingival scaling and root planing/high-throughput sequencing分类
医药卫生引用本文复制引用
赵红,王新林,吕治,王冬青,苏建荣..基于高通量测序技术研究慢性牙周炎患者龈下刮治和根面平整术治疗前后龈下菌群的变化[J].国际口腔医学杂志,2017,44(3):294-300,7.基金项目
首都临床特色应用研究(Z141107006614005) （Z141107006614005）
北京口腔医院学科建设基金(13-09-17)This study was supported by Beijing Municipal Science & Technology Commission(Z141107006614005) and Discipline Construction Fund of Beijing Stomatological Hospital(13-09-17). （13-09-17）
