广西医科大学学报2017，Vol.34Issue(4)：504-509,6.DOI:10.16190/j.cnki.45-1211/r.2017.04.006
脑梗死合并细菌性肺炎大鼠模型的建立与评价
Establishment and evaluation of rat model of cerebral infarction complicated with bacterial pneumonia
摘要
Abstract
Objective:To evaluate three models of cerebral infarction complicated with bacterial pneumonia in rats.Methods:The rats were subjected to cerebral ischemia by using modified Longa thread embolism method,and then were infected with Pseudomonas aeruginosa via nasal drip (group D),oral endotracheal intubation (group K) and intratracheal injection (group Q),in order to establish different models of cerebral infarction complicated with bacterial pneumonia.The area of cerebral infarction,white blood cells (WBC) counts and neutrophilic granulocyte percentage (MEUT) were compared among the three groups.Results:The WBC counts and MEUT were notably elevated at on day 3 and 7 after modeling in group D (P ＜0.05 or P ＜0.01).The WBC counts and MEUT were also increased on day 1,3 and 7 after modeling in group K and group Q (P ＜0.05 orP ＜0.01).The inflammatory changes of lung tissue were found in all groups of rats.Conclusion:The cerebral infarction complicated with bacterial pneumonia model established by the modified Longa method and intratracheal injection of bacteria was satisfactory,with the highest success rate,simple operation and good reproducibility.Thus it was suitable for the follow-up study.关键词
大鼠模型/脑梗死合并细菌性肺炎/改良线栓法/气管注射法Key words
rat model/cerebral infarction complicated with bacterial pneumonia/modified Longa thread embolism method/intratracheal injection分类
医药卫生引用本文复制引用
郑雪嘉,吕军影,李凯,刘双燕,胡谋波..脑梗死合并细菌性肺炎大鼠模型的建立与评价[J].广西医科大学学报,2017,34(4):504-509,6.基金项目
国家中医药管理局全国名老中医药专家传承工作室建设基金资助项目(国中医药人教发[2014]20号) （国中医药人教发[2014]20号）
广西医疗卫生适宜技术研究与开发基金资助项目(No.S201414-07) （No.S201414-07）
