广西医科大学学报2017，Vol.34Issue(4)：510-513,4.DOI:10.16190/j.cnki.45-1211/r.2017.04.007
白介素17A对口腔黏膜上皮细胞抗念珠菌黏附及β-防御素-2表达的影响
Effect of interleukin 17A on the expression of β-defensin-2 and the adherence of Candida albicans to human oral mucosal epithelial cells
摘要
Abstract
Objective:To investigate the effect of interleukin 17A (IL-17A) on the expression of β-defensin-2 (hBD-2) and the adherence of C.albicans to human oral mucosal epithelial cells (OMECs).Methods:OMECs was divided into 4 groups:a control group,an only recombinant human IL-17A (rhIL-17A) intervention group,a model group in which the cells were infected with C.albicans (1 ∶ 10 ratio),and an experimental group in which the cells were infected with C.albicans and treated with different concentrations (1,10,100 and 1,000 μg/L) of rhIL-17A.The mRNA and protein levels of hBD-2 were detected by fluorescent quantitative PCR (FQ-PCR) and enzyme-linked immuno sorbent assay (ELISA),respectively.Results:C,albicans adherence rate in the experimental group was a little lower than that in the model group (P ＞0.05).Different concentrations of rhIL-17A could up-regulate hBD-2 expression in OMECs,and 100 μtg/L was the highest (P ＜0.01).Treated with 100 μg/L rhIL-17A for 12 h,24 h and 48 h lead to markedly increased hBD-2 mRNA and protein levels in OMECs,but 48 h was the highest (P ＜0.05 or P ＜0.01).Conclusion:IL-17A could induce the hBD-2 expression in OMECs,whereas had no effect on the adherence of C.albicans to OMECs.关键词
重组人白介素17A/人β-防御素-2/白色念珠菌/人口腔黏膜上皮细胞Key words
recombinant human IL-17A/human β-defensin-2/Candida albicans/human oral mucosal epithelial cells分类
医药卫生引用本文复制引用
方梅飞,蒋兰岚,罗琴,林小洁,曾启新,陶人川..白介素17A对口腔黏膜上皮细胞抗念珠菌黏附及β-防御素-2表达的影响[J].广西医科大学学报,2017,34(4):510-513,4.基金项目
国家自然科学基金资助项目(No.81260167) （No.81260167）
