海南医学院学报2017，Vol.23Issue(7)：875-878,4.DOI:10.13210/j.cnki.jhmu.20170406.053
葛根素调节成骨细胞体外增殖及相关信号通路表达改变的实验研究
Experimental study on the regulating effect of puerarin on osteoblast proliferation in vitro and related signal pathway expression
摘要
Abstract
Objective: To study the regulating effect of puerarin on osteoblast proliferation in vitro and related signal pathway expression.Methods: Mouse osteoblasts MC353-E1 were cultured and divided into the puerarin group that were treated with 10 μg/mL puerarin and the control group that were treated with medium without drugs or serum.Before treatment as well as 12 h, 24 h and 48 h after treatment, MTS kits were used to determine the cell proliferation activity, and enzyme-linked immunosorbent assay kits were used to determine the expression of PI3K/AKT/FoxO signal pathway molecules and apoptosis genes.Results: On 12 h, 24 h and 48 h after treatment, the cell viability of puerarin group were significantly higher than those of control group, and p-PI3K, p-AKT, p-FoxO1 and p-FoxO3a expression were significantly higher than those of control group while Bmi, Bax, CytC, AIF and Caspase-3 expression were significantly lower than those of control group;puerarin had time-dependent regulating effect on the cell viability as well as p-PI3K, p-AKT, p-FoxO1, p-FoxO3a, Bmi, Bax, CytC, AIF and Caspase-3 expression.Conclusions: Puerarin can promote osteoblast proliferation through PI3K/AKT/FoxO signal pathways.关键词
骨质疏松/成骨细胞/葛根素/增殖/凋亡/信号通路Key words
Osteoporosis/Osteoblast/Puerarin/Proliferation/Apoptosis/Signaling pathway分类
医药卫生引用本文复制引用
胡洪生,黄伟,孙杰..葛根素调节成骨细胞体外增殖及相关信号通路表达改变的实验研究[J].海南医学院学报,2017,23(7):875-878,4.基金项目
2015年湖北省教育厅中青年人才项目(B20151901) （B20151901）
It was supported by the Young Talent Project of Hubei Education Department in 2015 (No.B20151901). （No.B20151901）
