解放军医药杂志2017，Vol.29Issue(3)：5-9,5.DOI:10.3969/j.issn.2095-140X.2017.03.002
类风湿关节炎贫血大鼠模型的建立及评价
Establishment and Evaluation of Anemia Rat Models Associated by Rheumatoid Arthritis
摘要
Abstract
Objective To establish rat models of rheumatoid arthritis and anemia (RA-A) to provide a good animal model for further study on pathogenesis and treatment of RA-A.Methods A total of 30 male Wistar rats with 150-170g body weight were randomly divided into control,model Ⅰ and model Ⅱ groups (n =10 for each group).Control group was injected with 0.625 ml/kg physiological saline at the left hind toe;model Ⅰ group was injected with 1.5 ml solution of 2.5 mg/ml Ⅱ collagen and 5 mt freund's complete adjuvant emulsion of 0.625 ml/kg at the same injection site with control group;model Ⅱ group was intramuscularly injected with 1.25 μg/kg tumor necrosis factor-α (TNF-α) at left muscles of thigh on the basis of treatment for model Ⅰ group.Values of red blood cell count (RBC),hemoglobin (HGB),degree of joint swelling and rat weight at different time points after intervention were respectively detected.Contents of serum interleukin-6 (IL-6),IL-1,TNF-α,interferon-γ (IFN-γ) and histopathological changes of articular cartilage in rats were also detected at the 39th days after model establishment.Results At the 2nd,5th,10th,17th and 39th days after model establishment,values of weight were significantly lower (P ＜ 0.01),while degree values of joint swelling were significantly higher in model Ⅰ and Ⅱ groups than those in control group (P ＜ 0.01).At the 10th,17th and 39th days after model establishment,RBC and HGB values in model Ⅱ group were significantly lower than those in control and model Ⅰ groups (P ＜0.01),and the values in model Ⅰ group were significantly lower than those in control group (P ＜0.01).At the 39th day after model establishment,IL-6,IL-1,TNF-α and IFN-γ contents in model Ⅱ group were significantly higher than those in control and model Ⅰ groups (P ＜ 0.01),and the contents in model group Ⅰ were significantly higher than those in control group (P ＜ 0.01).Histopathological damage degree of articular cartilage in model Ⅱ group was severe than those in control and model Ⅰ groups.Conclusion RA-A rat models can be successfully obtained by the method in this study,and it is an ideal model for studying pathogenesis and treatment of RA-A.关键词
关节炎,类风湿/贫血/大鼠,Wistar/肿瘤坏死因子-α/模型,动物Key words
Arthritis, rheumatoid/Anemia/Rats, Wistar/Tumor necrosis factor-α/Models, animal分类
医药卫生引用本文复制引用
赵志勇,王勇,马玉琛,王澎澎,李长云,张培..类风湿关节炎贫血大鼠模型的建立及评价[J].解放军医药杂志,2017,29(3):5-9,5.基金项目
“十一五”军队中医药重大临床攻关课题(2006011003) （2006011003）
解放军252医院院管课题(2013252yy56) （2013252yy56）
